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home  products  P70S6K, Active (R21-10H) Catalog Number Pack Size Price (US) R21-10H-05 5 μg  $195  R21-10H-10 10 μg  $285  R21-10H-20 20 μg  $495    Product Description     Data Sheets     Related Products     Description: Recombinant full-length human p70S6K was expressed by baculovirus in Sf9 insect cells using a N-terminal His tag.   Genbank Number: NM_003161   Sequence: Full-length   Expression System: Sf9 insect cells using baculovirus   Tag: N-terminal His tag   Species: Human Storage and Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Applications: Kinase Assay, Western Blot Molecular Weight: ~76 kDa Gene Aliases : S6K; PS6K; S6K1; STK14A; RPS6KB1; p70-alpha; p70(S6K)-alpha Scientific Background: p70S6K is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues (1). p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6k activity undergoes changes in the cell cycle and increases 20-fold in G1 cells released from G0 (2). p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as threonine 389 a site phosphorylated by phosphoinositide-dependent kinase 1 (PDK-1). References:  1. Ferrari, S. et al: S6 phosphorylation and the p70s6k/p85s6k. Crit Rev Biochem Mol Biol. 1994;29(6):385-413. Review. 2. Edelmann, HM. Et al: Cell cycle regulation of p70 S6 kinase and p42/p44 mitogen-activated protein kinases in Swiss mouse 3T3 fibroblasts. J Biol Chem. 1996 Jan 12;271(2):963-71.   Specific Activity:  Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.   Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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