home
products
|
|
 |
| AMPK (A1/B1/G3), Active (P56-10H) |
|
|
|
| Catalog Number |
Pack Size |
Price (US) |
| P56-10H-05 |
5 μg |
$495
|
| P56-10H-10 |
10 μg |
$795
|
| P56-10H-20 |
20 μg |
$1195
|
|
|
|
|
|
|
|
|
|
|
|
|
Description: Recombinant full-length human AMPK (combination of A1/B1/G3 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tags. |
|
|
|
|
|
|
|
Genbank Number:
NM_006251
, NM_006253 , NM_017431
|
|
|
|
|
|
|
|
Sequence:
Full-length
|
|
|
|
|
|
|
|
Expression System:
Sf9 insect cells using baculovirus
|
|
|
|
|
|
|
|
Tag:
C-terminal His tags
|
|
|
|
|
|
|
|
Species:
Human
|
|
|
|
Storage and Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications: Kinase Assay, Western Blot
Molecular Weight: ~68kDa (A1), ~38kDa (B1), ~51kDa (G3)
Gene Aliases : Subunit A1: PRKAA1, MGC33776, MGC57364
Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785
Subunit G3: PRKAG3
Scientific Background: AMPK (A1/B1/G3) is a member of the AMPK family which are heterotrimeric proteins consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress (1). AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and hydroxy beta-methylglutaryl-CoA reductase. The γ subunit is dominantly expressed in skeletal muscle where it may play a key role in the regulation of energy metabolism (2).
References:
1. Viollet, B. et al: Physiological role of AMP-activated protein kinase (AMPK): insights from knockout mouse models. Biochem. Soc. Trans. 2003; 31; 216–219.
2. Cheung, P. C. F. et al: Characterization of AMP-activated protein kinase gamma-subunit isoforms and their role in AMP binding. Biochem. J. 346: 659-669, 2000.
 |
|
Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet. |
 |
|
Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
SAME QUALITY...
BETTER RATES
SignalChem provides all of its products in
custom pack sizes to meet your discovery needs.
more info |
|
|
|
|
|
| |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
RELIABLE SERVICE...
QUALITY RESULTS
SignalChem has extensive experience in the development
and production of high quality protein 'targets'. Expertise
includes: cloning, expression and activation of signaling proteins.
more info |
|
|
|
|
|
| |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
EFFICIENT PROCESS...
FAST TURNAROUND TIME
SignalChem understands that timelines are of critical
importance. We commit to providing our partners with a fully customized inclusive report within a 2-week time period.
more info |
|
|
|
|
|
| |
|
|
|
|
|
|
|
|
|
