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| AMPK (A1/B2/G1), Active (P50-10H) |
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| Catalog Number |
Pack Size |
Price (US) |
| P50-10H-05 |
5 μg |
$495
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| P50-10H-10 |
10 μg |
$795
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| P50-10H-20 |
20 μg |
$1195
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Description: Recombinant full-length human AMPK (combination of A1/B2/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tags. |
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Genbank Number:
NM_006251
, NM_005399 , NM_002733
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Sequence:
Full-length
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Expression System:
Sf9 insect cells using baculovirus
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Tag:
C-terminal His tags
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Species:
Human
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Storage and Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications: Kinase Assay, Western Blot
Molecular Weight: ~68kDa (A1), ~36kDa (B2), ~40kDa (G1)
Gene Aliases : Subunit A1: PRKAA1, MGC33776, MGC57364
Subunit B2: PRKAB2, MGC61468
Subunit G1: PRKAG1, AMPKG, MGC8666
Scientific Background: AMPK (A1/B2/G1) is a member of the AMPK family which are heterotrimeric proteins consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress (1). AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and hydroxy beta-methylglutaryl-CoA reductase. The subunit may be a positive regulator of AMPK activity and is highly expressed in skeletal muscle (2).
References:
1. Viollet, B. et al: Physiological role of AMP-activated protein kinase (AMPK): insights from knockout mouse models. Biochem. Soc. Trans. 2003; 31; 216–219.
2. Thornton, C. et al: Identification of a novel AMP-activated protein kinase beta subunit isoform that is highly expressed in skeletal muscle. J. Biol. Chem. 273: 12443-12450, 1998.
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Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet. |
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Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.
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SAME QUALITY...
BETTER RATES
SignalChem provides all of its products in
custom pack sizes to meet your discovery needs.
more info |
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RELIABLE SERVICE...
QUALITY RESULTS
SignalChem has extensive experience in the development
and production of high quality protein 'targets'. Expertise
includes: cloning, expression and activation of signaling proteins.
more info |
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EFFICIENT PROCESS...
FAST TURNAROUND TIME
SignalChem understands that timelines are of critical
importance. We commit to providing our partners with a fully customized inclusive report within a 2-week time period.
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