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| PI3K (p110β/p85α), Active (P28-10H) |
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Pack Size |
Price (US) |
| P28-10H-05 |
5 μg |
$135
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| P28-10H-10 |
10 μg |
$195
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| P28-10H-20 |
20 μg |
$345
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Description: Recombinant full-length human p110beta and human p85alpha were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins. |
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Genbank Number:
NM_006219
, NM_181523.
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Sequence:
Full-length
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Expression System:
Sf9 insect cells using baculovirus
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Tag:
N-terminal His tags
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Species:
Human
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Storage and Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications: Kinase Assay, Western Blot
Molecular Weight: ~111 kDa (p110β), ~86 kDa (p85α)
Gene Aliases : p110α: PIK3CB, PI3K, PIK3C1, PI3Kbeta, MGC133043, p110-BETA, DKFZp779K1237
p85α: PIK3R1, GRB1, p85-ALPHA
Scientific Background: The PI3K comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110β catalytic subunit (PIK3CB) plays a role in regulating the formation and stability of alpha-2B-beta-3 integrin adhesion bonds, which are necessary for shear force-induced platelet activation (1). In animal model of prostate tumor formation induced by the tumor suppressor PTEN loss, ablation of p110β impedes tumorigenesis with a concomitant diminution of AKT phosphorylation (2).
References:
1. Jackson, S. P. et al: PI 3-kinase p110-beta: a new target for antithrombotic therapy. Nature Med. 11: 507-514, 2005.
2. Jia, S.et al: Essential roles of PI(3)K-p110-beta in cell growth, metabolism and tumorigenesis. Nature 454: 776-779, 2008.
activity.gif) |
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Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet. |
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Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet. |
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SAME QUALITY...
BETTER RATES
SignalChem provides all of its products in
custom pack sizes to meet your discovery needs.
more info |
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RELIABLE SERVICE...
QUALITY RESULTS
SignalChem has extensive experience in the development
and production of high quality protein 'targets'. Expertise
includes: cloning, expression and activation of signaling proteins.
more info |
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EFFICIENT PROCESS...
FAST TURNAROUND TIME
SignalChem understands that timelines are of critical
importance. We commit to providing our partners with a fully customized inclusive report within a 2-week time period.
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