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home  products  Anti-DYKDDDDK (F51-63R) Catalog Number Pack Size Price (US) F51-63R-100 100 μg  $225    15% OFF   Product Description     Data Sheets     Related Products     Description: Rabbit Polyclonal Antibody Specificity: Recognizes the DYKDDDDK tag Cited Applications: WB Cross Reactivity: All proteins tagged with DYKDDDDK epitope Host: Rabbit Immunogen: DYKDDDDK peptide conjugated to KLH Formulation: TBS, 50% glycerol Purification: Affinity chromatography Stability: Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Scientific Background: The DYKDDDDK octapeptide is small, highly charged, and hydrophilic, making it useful across a wide variety of applications [1]. It is commonly employed in structural and functional studies where it is necessary to preserve the native confirmation and and/or activity of the protein [2]. References:  1. Jarvik, JW. and Telmer, CA: Epitope tagging, Annual Review of Genetics, 1998; 32, 601-618. 2. Larson DM. et al: Functional expression and biochemical characterization of an epitope-tagged connexin37. Mol Cell Biol Res Commun 3: 2000; 115-121. For specific information on a given lot, see related technical data sheet :    Sample Data :  Representative western blot with Anti-DYKDDDDK (1:5000) using 5 μg, and 15 μg of a positive cell lysate transfected with a DYKDDDDK tagged fusion protein.

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