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home  products  Anti-RFP (R46-61M) Catalog Number Pack Size Price (US) R46-61M-100 100 μg  $225    15% OFF   Product Description     Data Sheets     Related Products     Description: Mouse Monoclonal Antibody Specificity: Recognizes native and denatured forms of RFP protein. Cited Applications: WB Cross Reactivity: Western blot RFP and its variants tag-RFP, DeRed, mCherry and mOrange, etc Other RFP variants may also be detectable Host/Isotype/Clone#: Mouse, IgG1, RF5R Immunogen: RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugates Formulation: 10mM PBS, pH7.2 Purification: Affinity chromatography Stability: Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Scientific Background: RFP or red fluorescent protein from sea anemone Entacmaea quadricolor is a member of the green florescent protein family and is extensively used as a tag and reporter protein in molecular and cellular biology. RFP has a molecular weight of approximately 29 kDa and an excitation and emission maxima at 559 and 611 nm (1). RFP fluoresces over a wide pH range (4-10) with high quantum yield. Like other anthozoan fluorescent proteins, RFP forms tetramers at physiological concentrations. References:  1. Wiedenmann, J . et al: Red fluorescent protein eqFP611 and its genetically engineered dimeric variants. J Biomed Opt. 2005 Jan-Feb;10(1):14003.. For specific information on a given lot, see related technical data sheet:    Sample Data :  Representative western blot with Anti-RFP (1:1000) using 5 μl, 10 μl and 20 μl of 293 cell lysate transfected with a RFP protein.

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