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| Catalog Number |
Pack Size |
Price (US) |
| P14-10H-05 |
5 μg |
$195
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| P14-10H-10 |
10 μg |
$285
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| P14-10H-20 |
20 μg |
$495
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Description: Recombinant full-length human PDK1 was expressed by baculovirus in Sf9 insect cells using a N-terminal His tag. |
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Genbank Number:
NM_002613
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Sequence:
Full-length
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Expression System:
Sf9 insect cells using baculovirus
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Tag:
N-terminal His tag
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Species:
Human
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Storage and Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications: Kinase Assay, Western Blot
Molecular Weight: ~67 kDa
Gene Aliases : PRO0461; PDPK1; MGC20087; MGC35290; PkB-like; PkB-like 1
Scientific Background: PDK1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns(3,4,5)P3 or PtdIns(3,4)P2 (1). PDK1 then activates protein kinase B (PKB) which, in turn, inactivates glycogen synthase kinase-3 (GSK3). The phosphorylation of other proteins by PKB and GSK3 is likely to mediate many of the intracellular actions of insulin. Thus, PDK1 plays a key role in mediating many of the actions of the second messenger(s) PtdIns(3,4, 5)P3 and/or PtdIns(3,4)P2. The human PDK1 is a 556-residue monomeric enzyme comprising of a catalytic domain that is most similar to the PKA, PKB and PKC subfamily of protein kinases.
References:
1. Cohen, P. et al: PDK1, one of the missing links in insulin signal transduction? FEBS Letter. 1997 Jun 23;410(1):3-10. Review.
2. Alessi, DR. et al: Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase B alpha. Curr Biol. 1997 Apr 1;7(4):261-9.
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Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet. |
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Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.
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SAME QUALITY...
BETTER RATES
SignalChem provides all of its products in
custom pack sizes to meet your discovery needs.
more info |
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RELIABLE SERVICE...
QUALITY RESULTS
SignalChem has extensive experience in the development
and production of high quality protein 'targets'. Expertise
includes: cloning, expression and activation of signaling proteins.
more info |
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EFFICIENT PROCESS...
FAST TURNAROUND TIME
SignalChem understands that timelines are of critical
importance. We commit to providing our partners with a fully customized inclusive report within a 2-week time period.
more info |
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