home
products
categories
|
|
 |
|
|
|
| Catalog Number |
Pack Size |
Price (US) |
| M13-11G-05 |
5 μg |
$195
|
| M13-11G-10 |
10 μg |
$285
|
| M13-11G-20 |
20 μg |
$495
|
|
|
|
|
|
|
|
|
|
|
|
|
Description: Recombinant human ASK1(649-946) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. |
|
|
|
|
|
|
|
Genbank Number:
NM_005923
|
|
|
|
|
|
|
|
Sequence:
649-946
|
|
|
|
|
|
|
|
Expression System:
Sf9 insect cells using baculovirus
|
|
|
|
|
|
|
|
Tag:
N-terminal GST tag
|
|
|
|
|
|
|
|
Species:
Human
|
|
|
|
Storage and Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications: Kinase Assay, Western Blot
Molecular Weight: ~60 kDa
Gene Aliases : MAP3K5; MEKK5; MAPKKK5
Scientific Background: ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6. Overexpression of ASK1 induces apoptotic cell death, and ASK1 is activated in cells treated with tumor necrosis factor-alpha (1). ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway. After activation by TRAF2, ASK1 activates MKK4, which in turn activates JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation (2).
References:
1. Ichijo, H. et al: Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science 275: 90-94, 1997.
2. Nishitoh, H. et al: ASK1 is essential for JNK/SAPK activation by TRAF2. Molec. Cell 2: 389-395, 1998.
 |
|
Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet. |
 |
|
Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
SAME QUALITY...
BETTER RATES
SignalChem provides all of its products in
custom pack sizes to meet your discovery needs.
more info |
|
|
|
|
|
| |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
RELIABLE SERVICE...
QUALITY RESULTS
SignalChem has extensive experience in the development
and production of high quality protein 'targets'. Expertise
includes: cloning, expression and activation of signaling proteins.
more info |
|
|
|
|
|
| |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
EFFICIENT PROCESS...
FAST TURNAROUND TIME
SignalChem understands that timelines are of critical
importance. We commit to providing our partners with a fully customized inclusive report within a 2-week time period.
more info |
|
|
|
|
|
| |
|
|
|
|
|
|
|
|
|
