home
products
categories
|
|
 |
| MAPKAPK5, Active (M42-10G) |
|
|
|
| Catalog Number |
Pack Size |
Price (US) |
| M42-10G-05 |
5 μg |
$195
|
| M42-10G-10 |
10 μg |
$285
|
| M42-10G-20 |
20 μg |
$495
|
|
|
|
|
|
|
|
|
|
|
|
|
Description: Recombinant full-length human MAPKAPK5 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. |
|
|
|
|
|
|
|
Genbank Number:
NM_003668
|
|
|
|
|
|
|
|
Sequence:
Full-length
|
|
|
|
|
|
|
|
Expression System:
Sf9 insect cells using baculovirus
|
|
|
|
|
|
|
|
Tag:
N-terminal GST tag
|
|
|
|
|
|
|
|
Species:
Human
|
|
|
|
Storage and Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications: Kinase Assay, Western Blot
Molecular Weight: ~79 kDa
Gene Aliases : PRAK
Scientific Background: MAPKAPK5 is a member of the serine/threonine kinase family that responds to cellular stress and proinflammatory cytokines. MAPKAPK5 is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta (1). MAPKAPK5 is activated in HeLa cells in response to cellular stress and proinflammatory cytokines. MAPKAPK5 activity is regulated by p38-alpha and p38-beta both in vitro and in vivo, and thr-182 is the regulatory phosphorylation site of MAPKAPK5 (2). In vitro, MAPKAPK5 kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites.
References:
1. New, L.; Jiang, Y. et al: PRAK, a novel protein kinase regulated by the p38 MAP kinase. EMBO J. 17: 3372-3384, 1998.
2. Ni, H.; Wang. et al: MAPKAPK5, a novel mitogen-activated protein kinase (MAPK)-activated protein kinase, is a substrate of the extracellular-regulated kinase (ERK) and p38 kinase. Biochem. Biophys. Res. Commun. 243: 492-496, 1998.
activity.gif) |
|
Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet. |
gel.gif) |
|
Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet. |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
SAME QUALITY...
BETTER RATES
SignalChem provides all of its products in
custom pack sizes to meet your discovery needs.
more info |
|
|
|
|
|
| |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
RELIABLE SERVICE...
QUALITY RESULTS
SignalChem has extensive experience in the development
and production of high quality protein 'targets'. Expertise
includes: cloning, expression and activation of signaling proteins.
more info |
|
|
|
|
|
| |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
EFFICIENT PROCESS...
FAST TURNAROUND TIME
SignalChem understands that timelines are of critical
importance. We commit to providing our partners with a fully customized inclusive report within a 2-week time period.
more info |
|
|
|
|
|
| |
|
|
|
|
|
|
|
|
|
