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| AMPK (A2/B1/G1), Active (P48-10H) |
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| Catalog Number |
Pack Size |
Price (US) |
| P48-10H-05 |
5 μg |
$495
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| P48-10H-10 |
10 μg |
$795
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| P48-10H-20 |
20 μg |
$1195
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Description: Recombinant full-length human AMPK (combination of A2/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tags. |
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Genbank Number:
NM_006252
, NM_006253 , NM_002733
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Sequence:
Full-length
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Expression System:
Sf9 insect cells using baculovirus
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Tag:
C-terminal His tags
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Species:
Human
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Storage and Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications: Kinase Assay, Western Blot
Molecular Weight: ~69kDa (A2), ~38kDa (B1), and ~40kDa (G1).
Gene Aliases : Subunits A2: PRKAA2, AMPK, AMPK2, PRKAA
Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785
Subunit G1: PRKAG1, AMPKG, MGC8666
Scientific Background: AMPK (A2/B1/G1) plays a key role in insulin signaling pathway and is a major therapeutic target for the treatment of diabetes (1). AMPK is viewed as a fuel sensor for glucose and lipid metabolism by modulating the activity of the autonomous nervous system in vivo. Short-term overexpression of a constitutively active form of AMPK in the liver leads to mild hypoglycemia and fatty liver due to increased fatty acid utilization (2).
References:
1. Viollet, B. et al: Physiological role of AMP-activated protein kinase (AMPK): insights from knockout mouse models. Biochem. Soc. Trans. 2003; 31; 216–219.
2. Foretz, M. et al: Short-term overexpression of a constitutively active form of AMP-activated protein kinase in the liver leads to mild hypoglycemia and fatty liver. Diabetes, 2005; 54 (5);1331-1339.
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Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet. |
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Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.
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SAME QUALITY...
BETTER RATES
SignalChem provides all of its products in
custom pack sizes to meet your discovery needs.
more info |
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RELIABLE SERVICE...
QUALITY RESULTS
SignalChem has extensive experience in the development
and production of high quality protein 'targets'. Expertise
includes: cloning, expression and activation of signaling proteins.
more info |
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EFFICIENT PROCESS...
FAST TURNAROUND TIME
SignalChem understands that timelines are of critical
importance. We commit to providing our partners with a fully customized inclusive report within a 2-week time period.
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