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| CDK9/CyclinK, Active (C40-10G) |
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Pack Size |
Price (US) |
| C40-10G-05 |
5 μg |
$195
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| C40-10G-10 |
10 μg |
$285
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| C40-10G-20 |
20 μg |
$495
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Description: Recombinant full-length human CDK9 and CyclinK were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. |
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Genbank Number:
NM_001261
, NM_003858
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Sequence:
Full-length
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Expression System:
Sf9 insect cells using baculovirus
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Tag:
N-terminal GST tags
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Species:
Human
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Storage and Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications: Kinase Assay, Western Blot
Molecular Weight: ~68 kDa and CyclinK~67 kDa
Gene Aliases : CDC2L4, C-2k, TAK, PITALRE; CCNK, CPR4, MGC9113
Scientific Background: CDK9/CyclinK is a member of the cyclin-dependent protein kinase (CDK) family. CDK9 is closely related to cdc28 and cdc2 and is an important regulator of the cell cycle (1). CDK9 is a component of the multiprotein complex TAK/P-TEFβ. CDK9 can modulate RNA polymerase II-directed transcription by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. CDK9 forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. CDK9 also interacts with the HIV-1 Tat protein which suggested a possible involvement of this protein in AIDS (2).
References:
1. Yang, Z. et al: The 7SK small nuclear RNA inhibits the CDK9/cyclin T1 kinase to control transcription. Nature, 2001; 414: 317-322.
2. Bullrich, F. et al: Chromosomal mapping of members of the cdc2 family of protein kinases, cdk3, cdk6, PISSLRE, and PITALRE, and a cdk inhibitor, p27-Kip1, to regions involved in human cancer. Cancer Res. 1995; 55: 1199-1205.
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Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet. |
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Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet. |
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SAME QUALITY...
BETTER RATES
SignalChem provides all of its products in
custom pack sizes to meet your discovery needs.
more info |
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RELIABLE SERVICE...
QUALITY RESULTS
SignalChem has extensive experience in the development
and production of high quality protein 'targets'. Expertise
includes: cloning, expression and activation of signaling proteins.
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EFFICIENT PROCESS...
FAST TURNAROUND TIME
SignalChem understands that timelines are of critical
importance. We commit to providing our partners with a fully customized inclusive report within a 2-week time period.
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