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home  products  types  BRAF (V599E), Active (B08-13G) Catalog Number Pack Size Price (US) B08-13G-05 5 μg  $195  B08-13G-10 10 μg  $285  B08-13G-20 20 μg  $495    Product Description     Data Sheets     Related Products     Description: Recombinant human BRAF (416-end; V599E) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.   Genbank Number: NM_004333   Sequence: 416-end   Expression System: Sf9 insect cells using baculovirus   Tag: N-terminal GST tag   Species: Human Storage and Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Applications: Kinase Assay, Western Blot Molecular Weight: ~63 kDa Gene Aliases : BRAF1, RAFB1, B-raf 1, MGC126806, MGC138284 Scientific Background: BRAF is a member of the RAF family that is activated by members of the Ras family upon growth factor-induced stimulation. Active Ras can induce heterodimerization of cRaf and BRAF and this may explain the observed cooperativity of cRaf and BRaf in cells responding to growth factor signals (1). Activating mutations in the BRAF gene are present in a large percentage of human malignant melanomas and in a proportion of colon cancers. The vast majority of these mutations result in a valine to glutamic acid change at residue 599 within the activation segment of B-RAF (2). References:  1. Weber, C K. et al: Active Ras induces heterodimerization of cRaf and BRaf. Cancer Res. 2001 May 1;61(9):3595-8. 2. Mercer, K E. et al: Raf proteins and cancer: B-Raf is identified as a mutational target. Biochim Biophys Acta. 2003 Jun 5;1653(1):25-40.   Specific Activity:  Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.   Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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