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| CDK3/CyclinE1, Active (C30-10G) |
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Pack Size |
Price (US) |
| C30-10G-05 |
5 μg |
$195
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| C30-10G-10 |
10 μg |
$285
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| C30-10G-20 |
20 μg |
$495
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Description: Recombinant full-length human CDK3 and Cyclin E1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. |
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Genbank Number:
NM_001258
, NM_001238
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Sequence:
Full-length
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Expression System:
Sf9 insect cells using baculovirus
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Tag:
N-terminal GST tags
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Species:
Human
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Storage and Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications: Kinase Assay, Western Blot
Molecular Weight: ~60 kDa and CyclinE1 ~73 kDa
Gene Aliases : CDK3: None
CyclinE1: CCNE1, CCNE
Scientific Background: CDK3/CyclinE1is a member of the cyclin-dependent protein kinase family and promotes entry into S phase, in part by activating members of the E2F family of transcription factors. CDK3 can also associate with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. The CDK3 gene has been mapped to chromosomal location of 17q22-qter, telomeric to the BRCA1 gene by somatic cell hybrids analysis (1). The presence of a single point mutation in the CDK3 gene from several Mus musculus strains commonly used in the laboratory has been reported (2).
References:
1. Bullrich, F. et al: Chromosomal mapping of members of the cdc2 family of protein kinases, cdk3, cdk6, PISSLRE, and PITALRE, and a cdk inhibitor, p27-Kip1, to regions involved in human cancer. Cancer Res. 55: 1199-1205, 1995.
2. Ye, X. et al: A premature-termination mutation in the Mus musculus cyclin-dependent kinase 3 gene. Proc. Nat. Acad. Sci. 98: 1682-1686, 2001.
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Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet. |
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Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet. |
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SAME QUALITY...
BETTER RATES
SignalChem provides all of its products in
custom pack sizes to meet your discovery needs.
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RELIABLE SERVICE...
QUALITY RESULTS
SignalChem has extensive experience in the development
and production of high quality protein 'targets'. Expertise
includes: cloning, expression and activation of signaling proteins.
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EFFICIENT PROCESS...
FAST TURNAROUND TIME
SignalChem understands that timelines are of critical
importance. We commit to providing our partners with a fully customized inclusive report within a 2-week time period.
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