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home  products  types  CDK6/CyclinD3, Active (C35-10H) Catalog Number Pack Size Price (US) C35-10H-05 5 μg  $195  C35-10H-10 10 μg  $285  C35-10H-20 20 μg  $495    Product Description     Data Sheets     Related Products     Description: Recombinant full-length human CDK6 and CyclinD3 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.   Genbank Number: NM_001259 , NM_001760   Sequence: Full-length   Expression System: Sf9 insect cells using baculovirus   Tag: N-terminal His tags   Species: Human Storage and Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Applications: Kinase Assay, Western Blot Molecular Weight: ~40 kDa and CyclinD3 ~35 kDa Gene Aliases : PLSTIRE, MGC59692 /CCND3 Scientific Background: CDK6 is a member of the cyclin-dependent family of protein kinases that are important regulators of cell cycle progression. CDK6 activity is regulated by the D-type cyclins and members of the INK4 family of CDK inhibitors (1). The CDK6 kinase activity is detected in mid-G1 phase of the cell cycle and is responsible for the phosphorylation and regulation of the activity of tumor suppressor protein Rb. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivities in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein (2). References:  1. Meyerson, M. et al: Identification of G1 kinase activity for cdk6, a novel cyclin D partner. Molec. Cell. Biol. 14: 2077-2086, 1994. 2. Takaki, T. et al: Preferences for phosphorylation sites in the retinoblastoma protein of D-type cyclin-dependent kinases, Cdk4 and Cdk6, in vitro. J Biochem. 2005 Mar;137(3):381-6.   Specific Activity:  Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.   Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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