Search SignalChem:   1-866-9 KINASE (54-6273)

Products Discovery Services Promotions Distribution Support Corporate Ordering

home  products  types  CDK2/CyclinE1, Active (C29-18G) Catalog Number Pack Size Price (US) C29-18G-05 5 μg  $195  C29-18G-10 10 μg  $285  C29-18G-20 20 μg  $495    Product Description     Data Sheets     Related Products     Description: Recombinant full-length human CDK2 and CyclinE1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.   Genbank Number: NM_001798 , NM_001238   Sequence: Full-length   Expression System: Sf9 insect cells using baculovirus   Tag: N-terminal GST tags   Species: Human Storage and Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Applications: Kinase Assay, Western Blot Molecular Weight: ~58kDa and CyclinE1 ~73kDa Gene Aliases : CDK2: p33 (CDK2) CyclinE1: CCNE1, CCNE Scientific Background: CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including Cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B) (1). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition (2). References:  1. Levkau, B. et al: Cleavage of p21(Cip1/Waf1) and p27(Kip1) mediates apoptosis in endothelial cells through activation of Cdk2: role of a caspase cascade. Molec. Cell1: 553-563, 1998. 2. Huang, H. et al: CDK2-dependent phosphorylation of FOXO1 as an apoptotic response to DNA damage. Science314: 294-297, 2006.   Specific Activity:  Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.   Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Bulk Orders   SAME QUALITY... BETTER RATES SignalChem provides all of its products in custom pack sizes to meet your discovery needs. more info           Custom Proteins   RELIABLE SERVICE...                 QUALITY RESULTS SignalChem has extensive experience in the development and production of high quality protein 'targets'. Expertise includes: cloning, expression and activation of signaling proteins. more info           Profiling Services   EFFICIENT PROCESS...      FAST TURNAROUND TIME SignalChem understands that timelines are of critical importance. We commit to providing our partners with a fully customized inclusive report within a 2-week time period. more info

Contact SignalChem [toll free]: 1-866-9 KINASE (54-6273) [phone]: 1-604-232-4600 [fax]: 1-604-232-4601 [email]: info@signalchem.com

Products  |   Discovery Services  |   Promotions  |   Distribution  |   Support  |   Corporate  |   Ordering Site By: Irtus Software