home
products
types
|
|
 |
| AMPK (A1/B1/G1), Active (P47-10H) |
|
|
|
| Catalog Number |
Pack Size |
Price (US) |
| P47-10H-05 |
5 μg |
$495
|
| P47-10H-10 |
10 μg |
$795
|
| P47-10H-20 |
20 μg |
$1195
|
|
|
|
|
|
|
|
|
|
|
|
|
Description: Recombinant full-length human AMPK (combination of A1/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using C-terminal His tags. |
|
|
|
|
|
|
|
Genbank Number:
NM_006251
, NM_006253 , NM_002733
|
|
|
|
|
|
|
|
Sequence:
Full-length
|
|
|
|
|
|
|
|
Expression System:
Sf9 insect cells using baculovirus
|
|
|
|
|
|
|
|
Tag:
C-terminal His tags
|
|
|
|
|
|
|
|
Species:
Human
|
|
|
|
Storage and Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications: Kinase Assay, Western Blot
Molecular Weight: ~68kDa (A1), ~38kDa (B1), ~40kDa (G1)
Gene Aliases : Subunit A1: PRKAA1, MGC33776, MGC57364
Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785
Subunit G1: PRKAG1, AMPKG, MGC8666
Scientific Background: AMPK is a heterotrimer protein kinase consisting of an α catalytic subunit, and non-catalytic β and γ subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status (1). In response to cellular metabolic stresses, AMPK is activated and phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating biosynthesis of fatty acid and cholesterol (2).
References:
1. Minokoshi, Y. et al:AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus. Nature 428: 569-574, 2004.
2. Hardie, D G. et al: The AMP-activated protein kinase--fuel gauge of the mammalian cell Eur J Biochem. 1997 Jun 1;246(2):259-73.
 |
|
Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet. |
 |
|
Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
SAME QUALITY...
BETTER RATES
SignalChem provides all of its products in
custom pack sizes to meet your discovery needs.
more info |
|
|
|
|
|
| |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
RELIABLE SERVICE...
QUALITY RESULTS
SignalChem has extensive experience in the development
and production of high quality protein 'targets'. Expertise
includes: cloning, expression and activation of signaling proteins.
more info |
|
|
|
|
|
| |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
EFFICIENT PROCESS...
FAST TURNAROUND TIME
SignalChem understands that timelines are of critical
importance. We commit to providing our partners with a fully customized inclusive report within a 2-week time period.
more info |
|
|
|
|
|
| |
|
|
|
|
|
|
|
|
|
