Description: Rabbit Polyclonal Antibody

Specificity: Recognizes the AKT3 protein

Cited Applications: WB

Cross Reactivity: Western blot AKT3 from human and mouse cells

AKT3 from other species may also be detectable

Host: Rabbit

Immunogen: Protein Code: Q9Y243

Formulation: TBS, 50% glycerol

Purification: Affinity Chromatography

Stability: Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: AKT 3 or Protein Kinase B (PKB ) is a serine/threonine kinase that is a member of the AKTγ (PKBγB family. AKT 3 is activated in cells exposed to diverse stimuli such as hormones, growth factors, and extracellular matrix components (1). AKT 3 phosphorylates and regulates the function of many cellular proteins involved in processes that include cellular metabolism, survival/apoptosis, and proliferation. Recent evidence indicates that AKT 3 is frequently overexpressed in many types of human cancers including breast and prostate (2).

References: 
1. Coffer, PJ. et al: Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 1998 Oct 1; 335 (Pt 1): 1-13.

2. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.

		Sample Data:  Representative western blot (1:1000) with Anti-AKT3 using 20 ng of human full length recombinant AKT1, AKT2, and AKT3.

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