Description: Rabbit Polyclonal Antibody

Specificity: Recognizes the AMPKα1 protein

Cited Applications: WB

Cross Reactivity: Western blot human AMPKα1

AMPK1 from other species may also be detectable

Host: Rabbit

Immunogen: Synthetic peptide corresponding to amino acids 483-487 of human AMPKα

Formulation: PBS pH 7.4, 0.02% sodium azide, 50% glycerol

Purification: Affinity chromatography

Stability: Store at 4°C (add 0.1% NaN₃) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis (1). AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γ subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) (2) and via signaling pathways in response to adiponectin, leptin and CAMKKβ.

References: 
1. Hardie, G.D. The AMP-activated protein kinase pathway – new players upstream and downstream. J. Cell Sci. 2004;117: 5479–5487.

2. Kahn, B.B. et al. AMP-activated protein kinase: Ancient energy gauge provides clues to modern understanding of metabolism. Cell Metab; 2005: 1, 15–25.

		Sample Data:  Representative western blot with Anti-AMPK1α (1:1000) using 20 ng of human recombinant AMPK1 and AMPK2.

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