Specificity: Recognizes the PKCδ protein
Cited Applications: WB
Cross Reactivity: Western blot human and mouse PKCδ
PKCδ from other species may also be detectable
Host : Rabbit
Immunogen: Synthesized peptide derived from C-terminus of human PKCδ protein
Formulation: TBS, 50% glycerol
Purification: Affinity chromatography
Stability: Store at 4
oC (add 0.1% NaN
3) for several months, and at -20
oC for longer periods.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background: Protein kinase C delta (PKCδ) is a member of the protein kinase C (PKC) family of serine-threonine kinases. It is a 79 kDa protein kinase that shows strict dependence on the presence of phospholipids, but shows no activation by Ca2+ (1). Phosphatidylinositol is the most potent activator of PKC delta. Northern blot analysis indicated that PKC delta is widely distributed in almost all the tissues and is a major isoform of PKC expressed in hemopoietic cells (2). PKC delta is involved in fundamental cellular functions regulated by diacylglycerols and mimicked by phorbol esters.
References: 1. Leibersperger, H. et al: Immunological demonstration of a calcium-unresponsive protein kinase C of the delta-type in different species and murine tissues. Predominance in epidermis. J Biol Chem. 1991 Aug 5; 266(22):14778-84.
2. Mischak, H. et al: Mouse protein kinase C-delta, the major isoform expressed in mouse hemopoietic cells: sequence of the cDNA, expression patterns, and characterization of the protein. Biochemistry. 1991 Aug 13; 30(32):7925-31.
WB_a_gel.jpg) |
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Sample Data: Representative Western blot with Anti- PKCδ (1:1000) using 15ng of GST-tagged human recombinant PKCα, PKCβI, PKCβII, PKCγ, PKCδ, PKCε, PKCν, PKCθ, PKCμ, PKCυ, PKCζ, and PKCι. |
WB_b_gel.jpg) |
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Sample Data: Representative Western blot (1:1000) with PKCδ using 10 μg, 30μg, 50μg of 293 cell lysates. |
The Anti-PKC delta product can be utilized in the following research areas, but not limited to:
Apoptosis/Autophagy, Cardiovascular Disease, ERK/MAPK Pathway, Neurobiology, NfkB Pathway, PKA/PKC Pathway
| Product Type |
Product Name |
Catalog Number |
Application |
Pack Sizes |
| Enzymes |
PKC gamma, Active |
P66-10G |
Kinase Assay, Western Blot |
View PKC gamma, Active
|
| Enzymes |
PKC beta I, Active |
P62-10G |
Kinase Assay, Western Blot |
View PKC beta I, Active
|
| Enzymes |
PKC eta, Active |
P67-10G |
Kinase Assay, Western Blot |
View PKC eta, Active
|
| Enzymes |
PKC delta, Active |
P64-10G |
Kinase Assay, Western Blot |
View PKC delta, Active
|
| Enzymes |
PKC alpha, Active |
P61-18G |
Kinase Assay, Western Blot |
View PKC alpha, Active
|
| Enzymes |
PKC beta II, Active |
P63-10G |
Kinase Assay, Western Blot |
View PKC beta II, Active
|
| Signaling Reagents |
PKC Lipid Activator, 10x |
L51-39 |
Kinase Assay |
View PKC Lipid Activator, 10x
|
| Signaling Reagents |
PKCtide |
P15-58 |
Kinase Assay |
View PKCtide
|
| Enzymes |
PKC epsilon, Active |
P65-10G |
Kinase Assay, Western Blot |
View PKC epsilon, Active
|
| Enzymes |
PKC mu, Active |
P72-10G |
Kinase Assay, Western Blot |
View PKC mu, Active
|
| Enzymes |
PKC iota, Active |
P68-10G |
Kinase Assay, Western Blot |
View PKC iota, Active
|
| Enzymes |
PKC nu, Active |
P73-10G |
Kinase Assay, Western Blot |
View PKC nu, Active
|
| Enzymes |
PKC theta, Active |
P74-10G |
Kinase Assay, Western Blot |
View PKC theta, Active
|
| Enzymes |
PKC zeta, Active |
P75-10G |
Kinase Assay, Western Blot |
View PKC zeta, Active
|
| Antibodies |
Anti-PKC delta/ theta |
P64-63DR |
WB |
View Anti-PKC delta/ theta
|
| Antibodies |
Anti-PKC mu |
P72-63R |
WB |
View Anti-PKC mu
|
| Enzymes |
PKC alpha, Active |
P61-10G |
Kinase Assay, Western Blot |
View PKC alpha, Active
|
| Antibodies |
Anti-PKC theta |
P74-63R |
WB |
View Anti-PKC theta
|
| Antibodies |
Anti-PKC delta |
P64-63R |
WB |
View Anti-PKC delta
|
| Antibodies |
Anti-PKC zeta |
P75-63R |
WB |
View Anti-PKC zeta
|