: Recognizes the STAT5 protein
: Western blot of human STAT5
STAT5 from other species may also be detectable
: Protein Code: P42229
: TBS, 50% glycerol
: Affinity chromatography
: Store at 4°C (add 0.1% NaN3
) for several months, and at -20°C for longer periods.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
: STAT5 (signal transducers and activators of transcription 5) is a member of the STAT family and this protein is phosphorylated by receptor associated protein kinases in response to cytokines and growth factors (1). The differentiation of T helper (Th) cells is regulated by members of the STAT family of signaling molecules. In BCR-ABL-positive cells, STAT5 is constitutively activated by tyrosine phosphorylation. STAT5 activation results in upregulation of Bcl-XL and increased resistance to induction of apoptosis. STAT5 is involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6 beta-hydroxylase (2).
1. Wang, D. et al: Naturally occurring dominant negative variants of Stat5. Molec. Cell Biol. 16: 6141-6148, 1996.
2. A Subramanian et al: STAT 5 and NF-Y are involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6beta-hydroxylase. Nucleic Acids Res. 1998; 26(9): 2173–2178.
||Sample Data: Representative western blot with Anti-STAT5 (1:250) using 20 ng of GST tagged human recombinant STAT1β, STAT2, STAT3, STAT4, STAT5, and STAT6.
The Anti-STAT5 product can be utilized in the following research areas, but not limited to:
Apoptosis/Autophagy, Cancer, ERK/MAPK Pathway, Inflammation, JAK/STAT Pathway