Description: Rabbit Polyclonal Antibody

Specificity: Recognizes the STAT5 protein

Cited Applications: WB

Cross Reactivity: Western blot of human STAT5

STAT5 from other species may also be detectable

Host: Rabbit

Immunogen: Protein Code: P42229

Formulation: TBS, 50% glycerol

Purification: Affinity chromatography

Stability: Store at 4°C (add 0.1% NaN₃) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: STAT5 (signal transducers and activators of transcription 5) is a member of the STAT family and this protein is phosphorylated by receptor associated protein kinases in response to cytokines and growth factors (1). The differentiation of T helper (Th) cells is regulated by members of the STAT family of signaling molecules. In BCR-ABL-positive cells, STAT5 is constitutively activated by tyrosine phosphorylation. STAT5 activation results in upregulation of Bcl-XL and increased resistance to induction of apoptosis. STAT5 is involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6 beta-hydroxylase (2).

References: 
1. Wang, D. et al: Naturally occurring dominant negative variants of Stat5. Molec. Cell Biol. 16: 6141-6148, 1996.

2. A Subramanian et al: STAT 5 and NF-Y are involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6beta-hydroxylase. Nucleic Acids Res. 1998; 26(9): 2173–2178.

		Sample Data:  Representative western blot with Anti-STAT5 (1:250) using 20 ng of GST tagged human recombinant STAT1β, STAT2, STAT3, STAT4, STAT5, and STAT6.

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