Access to highly validated biological reagents is of paramount importance in research and drug discovery effort. Isoform-specific antibodies specifically recognize one of the many isoforms of a given protein in a cell and are purified using affinity chromatography incorporating the immobilized protein target onto a rigid matrix.
Isoform-specific antibodies are tested for sensitivity and specificity using various immunological techniques such as Western blot and immunoprecipitation. As an example, AKT exists as AKT1, AKT2 and AKT3 and the AKT3 isoform-specific antibody from SignalChem specifically recognizes only the AKT3 isoform and not AKT1 and AKT2.
Isoform-specific antibodies can be used for multiple applications such as western blotting, immunoprecipitation, immunocytochemistry, ELISA and other immunological technologies. Isoform-specific antibodies can also be beneficial in the treatment of various human diseases such as cancer, diabetes and neurological disorders where they can have impact on only the defective isoform of a protein target without impacting the other normal protein isoforms.
SignalChem offers a unique line of highly validated isoform specific antibodies that specifically recognize the intended target with the greatest affinity. All of these antibodies are counter-screened against the recombinant isoforms of the appropriate protein that SignalChem offers as a means of ensuring true specificity.