Description: Rabbit Polyclonal Antibody

Specificity: Recognizes acetylated lysine residues in proteins and peptide, including acetylated histone, acetylated BSA and acetylated MBP.

Cited Applications: WB, ELISA, IP, IHC, IF

Cross Reactivity: Pan-specific antibody. No cross-reactions detected to the non-acetylated proteins and peptides.

Host: Rabbit

Immunogen: Acetylated Lysine-KLH conjugates

Formulation: PBS, 50% glycerol, pH 7.

Purification: By affinity chromatography on an acetyl lysine agarose column

Stability: Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: Post-translational modification of proteins and peptides is a robust way to regulate function of existing proteins or peptides. Acetylation on lysine residues is one example of post-translational modification and is performed by a variety of acetyl transferases in the cell. Histone acetyl-transferases (HATs) are universal regulators of eukaryotic transcriptional activity and emerging therapeutic targets for human diseases (1). The Acetylated Lysine Antibody conjugated to HRP detects acetylation on lysine residues of proteins and peptides.

References: 
1. Mischerikow, N. et al: Targeted large-scale analysis of protein acetylation. Proteomics. 2011 Feb;11(4):571-89.

		Sample Data:  Representative western blot of the acetylated protein profile with Anti-Acetylated Lysine, HRP Conjugated (1:1000) using TSA-treated (A) and non-treated (B) human melanoma cells (MMRU) lysate.

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