: Recognizes proteins with tri-methylation on lysine residues (N-epsilon).
: WB, ELISA, IP, IHC, IF
: Pan-specific antibody. No cross-reactions detected with acetylated, mono-methylated and di-methylated proteins.
: Methylated lysine-KLH conjugates.
: PBS, 50% glycerol, pH7.
: By affinity chromatography on a tri-methyl lysine agarose column
: Store at 4o
C (add 0.1% NaN3
) for several months, and at -20o
C for longer periods.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
: Post-translational modification of proteins and peptides is a robust way to regulate function of existing proteins or peptides. Methylation on lysine residues is one example of post-translational modification and is performed by a variety of protein methyltransferases in the cell. C/EBPs are extensively modified by methylation of lysine side chains and this regulated methylation profoundly affects the activity of C/EBPs (1). The Methylated Lysine Antibody conjugated to HRP detects methylation on lysine residues in proteins and peptides.
1. Leutz, R. et al: Crosstalk between phosphorylation and multi-site arginine/lysine methylation in C/EBPs. Transcr. 2011 Jan;2(1):3-8.
||Sample Data: Representative western blot of the tri-methylated protein profile with Anti-Methylated Lysine (Tri- epsilon-N-methyl), HRP Conjugated (1:1000) using human melanoma cells (MMRU) lysate (A) and MMRU lysate plus additional N- epsilon-tri-methyl lysine peptide inhibitor (50 ng/ul) (B).
The Anti-Methylated Lysine (Tri- epsilon-N-methyl), HRP Conjugated product can be utilized in the following research areas, but not limited to:
Apoptosis/Autophagy, Cancer, Cell Cycle, Neurobiology