: Recognizes the β-actin protein
: Western blot β-actin from human and mouse cells
β-actin from other species may also be detectable
Host / Isotype / Clone#
: Mouse, IgG2b, BA3R
: Slightly modified human β-cytoplasmic actin (2-15aa) peptide
: 10mM PBS, pH7.2
: Affinity Chromatography
: Store at 4o
C (add 0.1% NaN3
) for several months, and at -20o
C for longer periods.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
: Actin, a ubiquitous protein in eukaryotes, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate contractile potentials for muscle cells (1).
1. Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48–55.
||Sample Data: Representative western blot with Anti-β-Actin (1:1000) using 3 μg, 10 μg, and 30 μg of 293 cell lysate.
The Anti- beta-Actin product can be utilized in the following research areas, but not limited to: