Description: Mouse Monoclonal Antibody

Specificity: Recognizes the β-actin protein

Cited Applications: WB

Cross Reactivity: Western blot β-actin from human and mouse cells

β-actin from other species may also be detectable

Host / Isotype / Clone#: Mouse, IgG2b, BA3R

Immunogen: Slightly modified human β-cytoplasmic actin (2-15aa) peptide

Formulation: 10mM PBS, pH7.2

Purification: Affinity Chromatography

Stability: Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: Actin, a ubiquitous protein in eukaryotes, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate contractile potentials for muscle cells (1).

References: 
1. Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48–55.

		Sample Data:  Representative western blot with Anti-β-Actin (1:1000) using 3 μg, 10 μg, and 30 μg of 293 cell lysate.

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