Description: Rabbit Polyclonal Antibody

Specificity: Recognizes the ADD1 protein

Cited Applications: WB

Cross Reactivity: Western blot ADD1 from human and mouse cells

ADD1 from other species may also be detectable

Host : Rabbit

Immunogen: Synthetic peptide corresponding to amino acids 724-728 of human ADD1 (P35611)

Formulation: PBS pH 7.4, 0.02% sodium azide, 50% glycerol

Purification: Affinity chromatography

Stability: Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: ADD1 or Adducin 1 is a heterodimeric protein associated with the erythrocyte membrane skeleton, which promotes binding of spectrin to actin, and is a substrate for PKC and PKA (1). Alpha- and gamma-adducins are ubiquitously expressed while beta-adducin is expressed at high levels in brain and hematopoietic tissues. ADD1 can completely block elongation and depolymerization at the fast-growing barbed ends of actin filaments in vitro and this suggests that adducin restrict actin filament length in erythrocytes (2).

References: 
1. Gardner, K. et.al: A new erythrocyte membrane-associated protein with calmodulin binding activity: identification and purification. J. Biol. Chem. 261: 1339-1348, 1986.

2. Kuhlman. et.al: A new functions for adducin: calcium/calmodulin-regulated capping of the barbed ends of actin filaments. J. Biol. Chem. 271: 7986-7991, 1996.

		Sample Data :  Representative western blot with Anti-ADD1 (1:1000) using 3 μg, 10 μg and 30 μg of HEK-293T cell lysate.

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