Description: Rabbit Polyclonal Antibody

Specificity: Recognizes the AOS1 protein

Cited Applications: WB

Cross Reactivity: Western blot AOS1 from human and mouse cells

AOS1 from other species may also be detectable

Host : Rabbit

Immunogen: Synthetic peptide of c-terminal amino acid sequence of human AOS1 (Q9UBE0)

Formulation: TBS, 50% glycerol

Purification: Affinity chromatography

Stability: Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: AOS1 encodes a member of the SUMO-activating enzyme for the sumoylation of proteins that regulates protein structure and intracellular localization. AOS1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins. Sumoylation is a 2-step reaction in which the SUA1/UBA2 dimer functions as an E1 enzyme and UBC9 functions as an E2 enzyme (1). AOS1 is required for sumoylation of UBA2 by epitope-tagged sentrin-1, sentrin-2 and sentrin-3 (2).

References: 
1. Okuma. et.al: In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2. Biochem. Biophys. Res. Commun. 254: 693-698, 1999.

2. Gong, L. et.al: Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex. FEBS Lett. 448: 185-189, 1999.

		Sample Data :  Representative western blot with Anti-AOS1 (1:5000) using 3 μg, 10 μg and 30 μg of HeLa cell lysate.

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