: Recognizes the PLCγ1 protein
: Western blot human PLCγ1
PLCγ1 from other species may also be detectable
: Synthetic peptide corresponding to amino acids 769-773 of human PLCγ1
: PBS, pH 7.4, 0.02% NaN3, 50% glycerol
: Affinity chromatography
: Store at 4o
C (add 0.1% NaN3
) for several months, and at -20o
C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
: PLCγ1 or phospholipase C gamma 1 plays an important role in the intracellular transduction of message from receptor-mediated tyrosine kinase activation. PLCγ1 catalyzes the formation of two second-messenger molecules inositol 1, 4, 5-trisphosphate and diacylglycerol from phosphatidylinositol 4, 5-bisphosphate. PLCγ1 is a major substrate for heparin-binding growth factor 1 activated tyrosine kinase. PLCγ1 inhibits growth factor receptor-mediated invasion of breast and prostate tumor cells and is a physiological guanine nucleotide exchange factor for the nuclear GTPase PIKE (1).
1. Ye, K. et al: Phospholipase C-gamma-1 is a physiological guanine nucleotide exchange factor for the nuclear GTPase PIKE. Nature 415: 541-544, 2002.
||Sample Data: Representative Western blot with Anti- PLCγ1 (1:1000) using 3 μg, 10 μg and 30 μg of Jurkat cell lysate.
The Anti-PLC gamma 1 product can be utilized in the following research areas, but not limited to:
Cancer, Cardiovascular Disease, Inflammation, Metabolic Disorder, Neurobiology, PKA/PKC Pathway