Description: Rabbit Polyclonal Antibody

Specificity: Recognizes the PLCγ1 protein

Cited Applications: WB

Cross Reactivity: Western blot human PLCγ1

PLCγ1 from other species may also be detectable

Host : Rabbit

Immunogen: Synthetic peptide corresponding to amino acids 769-773 of human PLCγ1

Formulation: PBS, pH 7.4, 0.02% NaN3, 50% glycerol

Purification: Affinity chromatography

Stability: Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: PLCγ1 or phospholipase C gamma 1 plays an important role in the intracellular transduction of message from receptor-mediated tyrosine kinase activation. PLCγ1 catalyzes the formation of two second-messenger molecules inositol 1, 4, 5-trisphosphate and diacylglycerol from phosphatidylinositol 4, 5-bisphosphate. PLCγ1 is a major substrate for heparin-binding growth factor 1 activated tyrosine kinase. PLCγ1 inhibits growth factor receptor-mediated invasion of breast and prostate tumor cells and is a physiological guanine nucleotide exchange factor for the nuclear GTPase PIKE (1).

References: 
1. Ye, K. et al: Phospholipase C-gamma-1 is a physiological guanine nucleotide exchange factor for the nuclear GTPase PIKE. Nature 415: 541-544, 2002.

		Sample Data:  Representative Western blot with Anti- PLCγ1 (1:1000) using 3 μg, 10 μg and 30 μg of Jurkat cell lysate.

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