Description: Rabbit Polyclonal Antibody

Specificity: Recognizes the UBC9 protein

Cited Applications: WB

Cross Reactivity: Western blot UBC9 from human and mouse cells

UBC9 from other species may also be detectable

Host: Rabbit

Immunogen: Synthetic peptide of c-terminal amino acid sequence of human UBC9 (P63279)

Formulation: TBS, 50% glycerol

Purification: Affinity chromatography

Stability: Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: UBC9 is a member of the E2 ubiquitin-conjugating enzyme family that is involved in ubiquitin-dependent protein degradation system (1). Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes and ubiquitin-protein ligases. UBC9 can function as a co-factor of PLAGL2, uncoupling its enzymatic activity to mediate PLAGL2 interactive SP-C promoter activity (2). UBC9 also plays an important role in the up-regulation of the bone morphogenetic protein signaling pathway.

References: 
1. Yasugi, T. et.al: Identification of the structural and functional human homolog of the yeast ubiquitin conjugating enzyme UBC9. Nucleic Acids Res. 24: 2005-2010, 1996.

2. Wang, Z.-Y. et.al: Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9. J. Biol. Chem. 271: 24811-24816, 1996.

		Sample Data :  Representative western blot with Anti-UBC9 (1:1000) using 3 μg, 10 μg and 30 μg of Jurkat cell lysate.

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