Description: Rabbit Polyclonal Antibody

Specificity: Recognizes the eIF4E protein

Cited Applications: WB

Cross Reactivity: Western blot eIF4E from human and mouse cells

eIF4E from other species may also be detectable

Host: Rabbit

Immunogen: Synthesized peptide corresponds to amino acid 209 of human eIF4E protein.

Formulation: PBS, PH7.4, 0.02% NaN3, 50% glycerol

Purification: Affinity Chromatography

Stability: Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. EIF4E and is the rate-limiting component in protein synthesis. Overexpression of EIF4E causes malignant transformation (1). Overexpression of EIF4E in transgenic mice causes a marked increase in tumorigenesis. EIF4E is a potential physiological substrate for MNK1 and MNK2. MNK1 interacts with the EIF4F complex via its interaction with the C-terminal region of EIF4G, not through a direct interaction with EIF4E (2).

References: 
1. Ruggero D, et al: The translation factor eIF-4E promotes tumor formation and cooperates with c-Myc in lymphomagenesis. Nature Med. 10: 484-486, 2004.

2. Waskiewicz A J, et al: Mitogen-activated protein kinases activate the serine/threonine kinases Mnk1 and Mnk2. EMBO J. 16: 1909-1920, 1997.

		Sample Data:  Representative western blot with Anti-eIF4E (1:1000) using 20 μg of Hela, 293, THP1 and 3T6 cell lysates.

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