: Recognizes the eIF4E protein
: Western blot eIF4E from human and mouse cells
eIF4E from other species may also be detectable
: Synthesized peptide corresponds to amino acid 209 of human eIF4E protein.
: PBS, PH7.4, 0.02% NaN3, 50% glycerol
: Affinity Chromatography
: Store at 4o
C (add 0.1% NaN3
) for several months, and at -20o
C for longer periods.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
: EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. EIF4E and is the rate-limiting component in protein synthesis. Overexpression of EIF4E causes malignant transformation (1). Overexpression of EIF4E in transgenic mice causes a marked increase in tumorigenesis. EIF4E is a potential physiological substrate for MNK1 and MNK2. MNK1 interacts with the EIF4F complex via its interaction with the C-terminal region of EIF4G, not through a direct interaction with EIF4E (2).
1. Ruggero D, et al: The translation factor eIF-4E promotes tumor formation and cooperates with c-Myc in lymphomagenesis. Nature Med. 10: 484-486, 2004.
2. Waskiewicz A J, et al: Mitogen-activated protein kinases activate the serine/threonine kinases Mnk1 and Mnk2. EMBO J. 16: 1909-1920, 1997.
||Sample Data: Representative western blot with Anti-eIF4E (1:1000) using 20 μg of Hela, 293, THP1 and 3T6 cell lysates.
The Anti-eIF4E product can be utilized in the following research areas, but not limited to:
Angiogenesis, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Inflammation, Neurobiology