Description: Rabbit Polyclonal Antibody

Specificity: Recognizes the p21CIP1 (CDKN1) protein

Cited Applications: WB

Cross Reactivity: Western blot human p21CIP1

p21CIP1 from other species may also be detectable

Host : Rabbit

Immunogen: Synthetic peptide corresponding to amino acids 143-147 of human p21CIP1

Formulation: PBS, pH7.4, 0.02%NaN3, 50% glycerol

Purification: Affinity Chromatography

Stability: Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: CIP1 (Cyclin-Dependent Kinase Inhibitor 1A) regulates cell cycle progression, terminal differentiation, and apoptosis (1). CIP1 was shown to be induced by p53 and to be a potent inhibitor of cyclin-dependent kinase (CDK) activity. DNA damage leads to increased expression of CIP1 in cyclin E-containing complexes and to an associated decrease in cyclin-dependent kinase activity. CIP1 is a critical downstream effector in the p53-specific pathway of growth control in mammalian cells (2).

References: 
1. el-Deiry, W S. et al: WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis. Cancer Res. 1994;54(5):1169-74

2. Wagner, M. et al: Cyclin-dependent kinase-inhibitor 1 (CDKN1A) in the squamous epithelium of the oropharynx: possible implications of molecular biology and compartmentation. Anticancer Res. 2001; 21(1A):333-45.

		Sample Data:  Representative Western blot with Anti-p21CIP1 (1:1000) using 20ng of GST-tagged human recombinant p21CIP1.

		Sample Data:  Representative Western blot with Anti-p21CIP1 (1:1000) using 3 μg, 10 μg and 30 μg of THP1cell lysate.

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