Description: Rabbit Polyclonal Antibody

Specificity: Anti-p70S6K antibody recognizes the p70S6K protein.

Cited Applications: WB

Cross Reactivity: p70S6K from Human, Mouse, Rabbit, and Rat

p70S6K from other species may also be detectable.

Host: Rabbit

Immunogen: C-ISKRPEHLRMNL peptide KLH conjugates

Formulation: PBS at pH 7.2, 50% glycerol, 0.1% Tween-20

Purification: Affinity purified by peptide C-ISKRPEHLRMNL on agarose from rabbit serum

Stability: Store at 4°C (add 0.1% NaN₃) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: p70S6K is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues (1). p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6K activity undergoes changes in the cell cycle and increases 20-fold in G1 cells released from G0 (2). p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as threonine 389 a site phosphorylated by phosphoinositide-dependent kinase 1 (PDK-1).

References: 
1. Ferrari, S. et al: S6 phosphorylation and the p70s6k/p85s6k. Crit Rev Biochem Mol Biol. Review. 1994;29(6):385-413.

2. Edelmann, HM. Et al: Cell cycle regulation of p70 S6 kinase and p42/p44 mitogen-activated protein kinases in Swiss mouse 3T3 fibroblasts. J Biol Chem. 1996 Jan 12;271(2):963-71.

		Sample Data:  Representative western blot from HeLa cell lysate using p70S6K antibody (1:1000).

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