: Recognizes the p70S6K protein
: Western blot p70S6K from human and mouse cells
p70S6K from other species may also be detectable
: Synthetic peptide corresponding to amino acids 409-413
of human p70S6K (protein accession number P23443)
: PBS pH 7.4, 0.02% sodium azide, 50% glycerol
: Affinity chromatography
: Store at 4o
C (add 0.1% NaN3
) for several months, and at -20o
C for longer periods.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
: p70S6K is responsible for the phosphorylation of 40S
ribosomal protein S6, and is ubiquitously expressed in
human adult tissues (1). p70S6K is activated by serum
stimulation and this activation is inhibited by wortmannin
and rapamycin. p70S6k activity changes during the cell
cycle, and increases 20-fold in G1 cells released from G0
(2). p70S6K activation requires sequential phosphorylation
at proline-directed residues in the putative autoinhibitory
pseudosubstrate domain, as well as threonine 389, a site
phosphorylated by phosphoinositide-dependent kinase 1
1. Ferrari, S. et al: S6 phosphorylation and the p70s6k/p85s6k.
Crit Rev Biochem Mol Biol. 1994;29(6):385-413. Review.
2. Edelmann, HM. Et al: Cell cycle regulation of p70 S6 kinase
and p42/p44 mitogen-activated protein kinases in Swiss
mouse 3T3 fibroblasts. J Biol Chem. 1996 Jan 12;271(2):963-71.
||Sample Data: Representative western blot with Anti-p70S6K (1:1000) using 5 ng
of activated His-tag human recombinant p70S6K.
||Sample Data: Representative western blot with Anti-p70S6K (1:1000) using 30 μg
of HeLa, HEK-293T, Jurkat, THP1, and 3T6 cell lysate.
The Anti-p70S6K product can be utilized in the following research areas, but not limited to:
AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Metabolic Disorder