Description: Goat Polyclonal Antibody

Specificity: Anti-Rabbit HRP conjugated secondary antibody recognizes rabbit IgG and IgM.

Cited Applications: WB, ELISA

Cross Reactivity: IgG and IgM from Rabbit

(Other rabbit immunoglobulins may also be detectable)

Host / Isotype / Clone#: Goat Polyclonal

Immunogen: Affinity purified rabbit immunoglobulins

Formulation: PBS at pH 7.2, 50% Glycerol

Purification: Affinity purified with rabbit immunoglobulins on agarose followed by adsorption with human serum albumin and human gamma globulins

Stability: Store at 4°C for several months, and at -20°C for longer periods; do not add NaN₃ as this will inhibit the HRP enzyme. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: Affinity purified rabbit immunoglobulins were used to raise goat antibodies specific to the rabbit IgG. The goat immune serum is subsequently affinity purified using immunoglobulins immobilized to agarose. The antibodies are then conjugated to horse radish peroxidase (HRP) by reductive amination in a 2:1 molar ratio. The HRP conjugated secondary antibody can recognize the primary immunoglobulin and give a signal in the presence of a chemiluminescent substrate. The signal can be detected using an electronic imager or autoradiography film (1).

References: 
1. Nakane PK, Pierce GB: Enzyme-labeled antibodies: preparation and application for localization of antigens, J Histochem Cytochem 1966, 14:929

		Sample Data:  Representative western blot (1:2000) from HeLa cell lysate using a rabbit pAb RSK1 antibody recognized by Anti-Rabbit HRP

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