Description: Mouse Monoclonal Antibody

Specificity: Anti-GST antibody recognizes the N-terminal or C-terminal GST-tagged fusion protein.

Cited Applications: WB

Cross Reactivity: All proteins tagged with GST

Host / Isotype / Clone#: Mouse, IgG2a, G2R

Immunogen: Purified GST

Formulation: 10mM PBS, pH 7.2

Purification: Purified by Protein A affinity chromatography from mouse ascites fluid

Stability: Store at 4°C (add 0.1% NaN₃) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: GST or glutathione S-transferase family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are used to create the so-called 'GST gene fusion system' (1). These proteins are capable of multiple reactions with a multitude of substrates. GST tag has the size of 220 amino acids, which is relatively big compared to other tags like the Myc- or the DYKDDDDK-tag that are quite small. GST can be used to purify and detect proteins of interest.

References: 
1. Beckett, G.J. and Hayes, J.D. Glutathione S-transferase measurements and liver disease in man. J. Clin. Biochem. Nutr. 1987. 2, 1-24.

		Sample Data:  Representative western blot (1:1000) using GST tagged fusion protein.

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