Description: Mouse Monoclonal Antibody

Specificity: Recognizes native and denatured forms of RFP protein.

Cited Applications: WB

Cross Reactivity: Western blot RFP and its variants tag-RFP, DeRed, mCherry and mOrange, etc
Other RFP variants may also be detectable

Host / Isotype / Clone#: Mouse, IgG1, RF5R

Immunogen: RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugates

Formulation: 10mM PBS, pH7.2

Purification: Affinity chromatography

Stability: Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: RFP or red fluorescent protein from sea anemone Entacmaea quadricolor is a member of the green florescent protein family and is extensively used as a tag and reporter protein in molecular and cellular biology. RFP has a molecular weight of approximately 29 kDa and an excitation and emission maxima at 559 and 611 nm (1). RFP fluoresces over a wide pH range (4-10) with high quantum yield. Like other anthozoan fluorescent proteins, RFP forms tetramers at physiological concentrations.

References: 
1. Wiedenmann, J . et al: Red fluorescent protein eqFP611 and its genetically engineered dimeric variants. J Biomed Opt. 2005 Jan-Feb;10(1):14003..

		Sample Data :  Representative western blot with Anti-RFP (1:1000) using 5 μl, 10 μl and 20 μl of 293 cell lysate transfected with a RFP protein.

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