In cell based studies, many proteins are expressed with 'tags' as these tags aid in establishing whether a target is expressed, at what level it is expressed and where it is localized. Furthermore, protein tags can aid in the purification of the target protein from crude cell lysates.
Tag antibodies developed against a variety of protein tags can be utilized for various applications. Tag antibody can be used to detect the expression of the recombinant protein as well as to purify them. Tag antibodies consisting of short sequences have been widely used in the study of protein expression in various systems.
Tag antibodies are useful reagents that easily identify, detect, or purify tag fusion proteins from cell lysates. They can also be used in western blotting, immunoprecipitation, immunocytochemistry, ELISA and other immunological technologies. Tag antibodies can be used in conjunction with anti-rabbit secondary antibodies, such as anti-rabbit HRP (horseradish peroxidase) that readily detect the proteins in assays such as western blotting.
SignalChem offers a wide range of tag antibodies that can be used to detect different tagged proteins by western blotting, immunoprecipitation, immunocytochemistry, ELISA and other immunological technologies.