Description: Full-length recombinant human HDAC3 was expressed by baculovirus in Sf9 insect cells using a C-terminal GST tag.

Storage, Stability, and Shipping: Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: HDAC Assay, Western Blot

Molecular Weight: ~83 kDa

Gene Aliases : HD3; RPD3; RPD3-2

Scientific Background: HDAC3 or Histone deacetylase 3 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex that represses transcription when tethered to a promoter. HDAC3 may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. HDAC3 can also down-regulate p53 function and thus modulate cell growth and apoptosis. Catalytic domain of HDAC4 can interact with HDAC3 via the transcriptional corepressor NCOR2 (1). RELA is a nonhistone substrate of HDAC3 and IKBA-dependent nuclear export of the HDAC3-deacetylated RELA replenishes the depleted cytoplasmic pool of latent NFKB-IKBA complexes (2).

References: 
1. Chen, L. et. al: Duration of nuclear NF-kappa-B action regulated by reversible acetylation. Science 293: 1653-1657, 2001.

2. Fischle, W. et al: Enzymatic activity associated with class II HDACs is dependent on a multiprotein complex containing HDAC3 and SMRT/N-CoR. Molec. Cell 9: 45-57, 2002.

		Specific Activity:  Sample Histone Deacetylase Activity Plot. For specific information on a given lot, see related technical data sheet.

		Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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