Description: Full-length recombinant human SIRT7 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Storage, Stability, and Shipping: Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: SIRT Assay

Molecular Weight: ~46 kDa

Gene Aliases: SIR2L7, MGC126840, MGC126842

Scientific Background: SIRT7 is a member of the class IV of sirtuin family of proteins which are homologs to the yeast Sir2 protein and play a role in cell differentiation, proliferation, apoptosis, metabolism, and senescence. SIRT7 associates with active rRNA genes and histones. Overexpression of SIRT7 increases pol I-mediated transcription whereas knockdown of SIRT7 or inhibition of its catalytic activity results in decreased association of pol I with rDNA and reduced pol I transcription (1). Depletion of SIRT7 stops cell proliferation and triggers apoptosis. SIRT7 deacetylates p53 and increases cellular resistance to cytotoxic and oxidative stress (2).

References: 
1. Ford, E. et al: Mammalian Sir2 homolog SIRT7 is an activator of RNA polymerase I transcription. Genes Dev. 20: 1075-1080, 2006.

2. Vakhrusheva, O. et al Sirt7 increases stress resistance of cardiomyocytes and prevents apoptosis and inflammatory cardiomyopathy in mice. Circ. Res. 102: 703-710, 2008.

		Specific Activity:  Sample SIRT Activity Plot. For specific information on a given lot, see related technical data sheet.

		Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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