AMPK (A1/B1/G1) Active Enzymes Active Kinases


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AMPK (A1/B1/G1), Active (P47-10H)
Catalog Number Pack Size Price (USD)  
AMPK (A1/B1/G1), Active P47-10H-05 5 ug $395   AMPK (A1/B1/G1), Active Add to Cart
AMPK (A1/B1/G1), Active P47-10H-10 10 ug $595   AMPK (A1/B1/G1), Active Add to Cart
AMPK (A1/B1/G1), Active P47-10H-BULK BULK CONTACT US  
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Bulk Pricing for AMPK (A1/B1/G1), Active

Product Data Sheet(s)




Description: Recombinant full-length human AMPK (combination of A1/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using C-terminal His tags.
Tag: His tags
Expression System: Sf9 insect cells using baculovirus
Species: Human
Sequence: Full length
Genbank Number: NM_006251 , NM_006253 , NM_002733
   

Storage, Stability, and Shipping: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Kinase Assay, Western Blot

Molecular Weight: ~68kDa (A1), ~38kDa (B1), ~40kDa (G1)

Gene Aliases : Subunit A1: PRKAA1, MGC33776, MGC57364 Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785 Subunit G1: PRKAG1, AMPKG, MGC8666

Scientific Background: AMPK is a heterotrimer protein kinase consisting of an α catalytic subunit, and non-catalytic β and γ subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status (1). In response to cellular metabolic stresses, AMPK is activated and phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating biosynthesis of fatty acid and cholesterol (2).

References: 
1. Minokoshi, Y. et al:AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus. Nature 428: 569-574, 2004.

2. Hardie, D G. et al: The AMP-activated protein kinase--fuel gauge of the mammalian cell Eur J Biochem. 1997 Jun 1;246(2):259-73.



  Specific Activity:  Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.


  Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.




AMPK (A1/B1/G1), Active Research Areas

The AMPK (A1/B1/G1), Active product can be utilized in the following research areas, but not limited to:

Apoptosis/Autophagy, Cancer, Cellular Stress, Metabolic Disorder, Ser/Thr Kinases



AMPK (A1/B1/G1), Active Related Products Found: Total 74 Related Products
Product Type Product Name Catalog Number Application Pack Sizes
Signaling Reagents Kinase Assay Buffer I K01-09 Kinase Assay View Kinase Assay Buffer I
Signaling Proteins STAT3 Protein S54-54G Kinase Assay, Western Blot View STAT3 Protein
Enzymes CAMKK2, Active C18-10G Kinase Assay, Western Blot View CAMKK2, Active
Signaling Proteins RAS Protein R05-30G Kinase Assay, Western Blot View RAS Protein
Enzymes MEK1 (EE), Active M02-12G Kinase Assay, Western Blot View MEK1 (EE), Active
Signaling Proteins MEK1, Unactive M02-14H Kinase Assay, Western Blot View MEK1, Unactive
Enzymes ERK1, Active M29-10U Kinase Assay, Western Blot View ERK1, Active
Signaling Proteins ERK1, Unactive M29-14U Kinase Assay, Western Blot View ERK1, Unactive
Enzymes MNK2, Active M55-10G Kinase Assay, Western Blot View MNK2, Active
Enzymes JNK1, Active M33-10G Kinase Assay, Western Blot View JNK1, Active
Signaling Proteins JNK1, Unactive M33-14G Kinase Assay, Western Blot View JNK1, Unactive
Signaling Proteins ERK2, Unactive M28-14G Kinase Assay, Western Blot View ERK2, Unactive
Enzymes AKT1, Active A16-10G Kinase Assay, Western Blot View AKT1, Active
Enzymes AKT2, Active A17-10G Kinase Assay, Western Blot View AKT2, Active
Enzymes AKT2, Active A17-10H Kinase Assay, Western Blot View AKT2, Active
Enzymes AKT3, Active A18-10G Kinase Assay, Western Blot View AKT3, Active
Enzymes GSK3 alpha, Active G08-10G Kinase Assay, Western Blot View GSK3 alpha, Active
Enzymes GSK3 beta, Active G09-10G Kinase Assay, Western Blot View GSK3 beta, Active
Enzymes PDK1, Active P14-10H Kinase Assay, Western Blot View PDK1, Active
Enzymes PKAc alpha, Active P51-10G Kinase Assay, Western Blot View PKAc alpha, Active
Enzymes PKAc beta, Active P52-10G Kinase Assay, Western Blot View PKAc beta, Active
Enzymes PKAc gamma, Active P53-10G Kinase Assay, Western Blot View PKAc gamma, Active
Enzymes PKC alpha, Active P61-10G Kinase Assay, Western Blot View PKC alpha, Active
Enzymes PKC alpha, Active P61-18G Kinase Assay, Western Blot View PKC alpha, Active
Enzymes PKC beta I, Active P62-10G Kinase Assay, Western Blot View PKC beta I, Active
Enzymes PKC beta II, Active P63-10G Kinase Assay, Western Blot View PKC beta II, Active
Enzymes PKC delta, Active P64-10G Kinase Assay, Western Blot View PKC delta, Active
Enzymes PKC epsilon, Active P65-10G Kinase Assay, Western Blot View PKC epsilon, Active
Enzymes PKC gamma, Active P66-10G Kinase Assay, Western Blot View PKC gamma, Active
Enzymes PKC eta, Active P67-10G Kinase Assay, Western Blot View PKC eta, Active
Enzymes PKC iota, Active P68-10G Kinase Assay, Western Blot View PKC iota, Active
Enzymes PKC mu, Active P72-10G Kinase Assay, Western Blot View PKC mu, Active
Enzymes PKC nu, Active P73-10G Kinase Assay, Western Blot View PKC nu, Active
Enzymes PKC zeta, Active P75-10G Kinase Assay, Western Blot View PKC zeta, Active
Enzymes p70S6K, Active R21-10H Kinase Assay, Western Blot View p70S6K, Active
Signaling Reagents AKT (PKB) Substrate A05-58 Kinase Assay View AKT (PKB) Substrate
Signaling Reagents AKT (SGK) Substrate A08-58 Kinase Assay View AKT (SGK) Substrate
Signaling Reagents Crosstide C51-58 Kinase Assay View Crosstide
Signaling Reagents PKA Substrate C01-58 Kinase Assay View PKA Substrate
Signaling Proteins AKT1, Unactive A16-14G Kinase Assay, Western Blot View AKT1, Unactive
Signaling Proteins AKT3, Unactive A18-14G Kinase Assay, Western Blot View AKT3, Unactive
Signaling Proteins GSK3 beta, Unactive G09-14G Kinase Assay, Western Blot View GSK3 beta, Unactive
Signaling Reagents S6K Substrate S05-58 Kinase Assay View S6K Substrate
Signaling Reagents GSK3 Substrate G50-58 Kinase Assay View GSK3 Substrate
Signaling Reagents PKCtide P15-58 Kinase Assay View PKCtide
Signaling Reagents Tyr-phosphopeptide-2 T70-58 Phosphatase Assay View Tyr-phosphopeptide-2
Enzymes PTPN6 (SHP1), Active P33-20G Phosphatase Assay, Western Blot View PTPN6 (SHP1), Active
Enzymes PTPN11 (SHP2), Active P38-21G Phosphatase Assay, Western Blot View PTPN11 (SHP2), Active
Enzymes CAMKK1, Active C17-10G Kinase Assay, Western Blot View CAMKK1, Active
Enzymes RAF1 (EE), Active R01-13G Kinase Assay, Western Blot View RAF1 (EE), Active
Enzymes MEK2, Active M03-10G Kinase Assay, Western Blot View MEK2, Active
Enzymes AMPK (A2/B1/G1), Active P48-10H Kinase Assay, Western Blot View AMPK (A2/B1/G1), Active
Enzymes PKC theta, Active P74-10G Kinase Assay, Western Blot View PKC theta, Active
Signaling Reagents 4EBP1 Protein E35-54H Western Blot View 4EBP1 Protein
Enzymes ERK2, Active M28-10G Kinase Assay, Western Blot View ERK2, Active
Enzymes AMPK (A1/B1/G2), Active P55-10H Kinase Assay, Western Blot View AMPK (A1/B1/G2), Active
Signaling Reagents SAMStide S07-58 Kinase Assay View SAMStide
Enzymes GSK3 beta, Active G09-10H Kinase Assay, Western Blot View GSK3 beta, Active
Enzymes MEK1, Active M02-10G Kinase Assay, Western Blot View MEK1, Active
Signaling Proteins STAT3 Protein S54-54H Kinase Assay, Western Blot View STAT3 Protein
Signaling Reagents AMP Solution A46-09 Kinase Assay View AMP Solution
Signaling Proteins MEK2, Unactive M03-14G Kinase Assay, Western Blot View MEK2, Unactive
Enzymes AMPK (A1/B1/G3), Active P56-10H Kinase Assay, Western Blot View AMPK (A1/B1/G3), Active
Enzymes AMPK (A1/B2/G1), Active P50-10H Kinase Assay, Western Blot View AMPK (A1/B2/G1), Active
Signaling Proteins BAX Protein B14-30G Western Blot View BAX Protein
Signaling Proteins AKT2, Unactive A17-15H Kinase Assay, Western Blot View AKT2, Unactive
Enzymes p70S6Kb, Active R22-10G Kinase Assay, Western Blot View p70S6Kb, Active
Signaling Proteins p70S6Kb, Unactive R22-14G Kinase Assay, Western Blot View p70S6Kb, Unactive
Antibodies Anti-AMPK alpha 1/2 P47-63BR WB View Anti-AMPK alpha 1/2
Antibodies Anti-AMPK alpha 1 P47-63R WB View Anti-AMPK alpha 1
Signaling Reagents AMARA Peptide A11-58 Kinase Assay View AMARA Peptide
Enzymes AMPK (A2/B2/G2), Active P54-10H Kinase Assay, Western Blot View AMPK (A2/B2/G2), Active
Enzymes AMPK (A2/B2/G1), Active P49-10H Kinase Assay, Western Blot View AMPK (A2/B2/G1), Active
Enzymes NUAK1, Active N19-10G Kinase Assay, Western Blot View NUAK1, Active


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Product Types
Enzymes
Enzymes Active Acetyltransferases Active Acetyltransferases
Enzymes Active Arginine Deiminases Active Arginine Deiminases
Enzymes Active Histone Deacetylases Active Histone Deacetylases
Enzymes Active Kinases Active Kinases
Enzymes Active Methyltransferase Active Methyltransferase
Enzymes Active Phosphatases Active Phosphatases
Enzymes Active Phosphodiesterases Active Phosphodiesterases
Extracellular Ligands
Extracellular Ligands Chemokines Chemokines
Extracellular Ligands Cytokines Cytokines
Extracellular Ligands Growth Factors Growth Factors
Signaling Proteins
Signaling Proteins Acetyl/Methyltransferase Proteins Acetyl/Methyltransferase Proteins
Signaling Proteins Adaptor Proteins Adaptor Proteins
Signaling Proteins Apoptosis Proteins Apoptosis Proteins
Signaling Proteins Arginine Deiminases Arginine Deiminases
Signaling Proteins Cell Cycle Proteins Cell Cycle Proteins
Signaling Proteins Cell Stress & Chaperone Proteins Cell Stress & Chaperone Proteins
Signaling Proteins Cellular Proteins Cellular Proteins
Signaling Proteins Deacetylase/Demethylase Proteins Deacetylase/Demethylase Proteins
Signaling Proteins Fructose Kinase Proteins Fructose Kinase Proteins
Signaling Proteins G-Proteins G-Proteins
Signaling Proteins Lysyl Oxidase Proteins Lysyl Oxidase Proteins
Signaling Proteins Methylcytosine Dioxygenase Proteins Methylcytosine Dioxygenase Proteins
Signaling Proteins Microtubule/Actin Associated Proteins Microtubule/Actin Associated Proteins
Signaling Proteins Tau Proteins Tau Proteins
Signaling Proteins Transcription Proteins Transcription Proteins
Signaling Proteins Ubiquitin Proteins Ubiquitin Proteins
Signaling Proteins Unactive Kinases Unactive Kinases
Signaling Reagents
Signaling Reagents Assay Reagents Assay Reagents
Signaling Reagents Oligo Substrates Oligo Substrates
Signaling Reagents Peptide Substrates Peptide Substrates
Signaling Reagents Protein Substrates Protein Substrates
Antibodies
Antibodies Isoform Specific Antibodies Isoform Specific Antibodies
Antibodies Modification Antibodies Modification Antibodies
Antibodies Phospho Specific Antibodies Phospho Specific Antibodies
Antibodies Primary Antibodies Primary Antibodies
Antibodies Secondary Antibodies Secondary Antibodies
Antibodies Tag Antibodies Tag Antibodies
Discovery Services
Compound Selective Profiling Compound Selective Profiling
Custom Protein Development Custom Protein Development

Research Categories
AKT/PKB Pathway AKT/PKB Pathway
Angiogenesis Angiogenesis
Apoptosis/Autophagy Apoptosis/Autophagy
Cancer Cancer
Cardiovascular Disease Cardiovascular Disease
Cell Cycle Cell Cycle
Cellular Stress Cellular Stress
Cytoplasmic Tyrosine Kinases Cytoplasmic Tyrosine Kinases
ERK/MAPK Pathway ERK/MAPK Pathway
Inflammation Inflammation
Invasion/Metastasis Invasion/Metastasis
JAK/STAT Pathway JAK/STAT Pathway
JNK/SAPK Pathway JNK/SAPK Pathway
Lipid Kinases Lipid Kinases
Metabolic Disorder Metabolic Disorder
Neurobiology Neurobiology
NfkB Pathway NfkB Pathway
p38 Pathway p38 Pathway
Phosphatases Phosphatases
Phosphodiesterases Phosphodiesterases
PKA/PKC Pathway PKA/PKC Pathway
Receptor Tyrosine Kinases Receptor Tyrosine Kinases
Ser/Thr Kinases Ser/Thr Kinases
WNT Signaling WNT Signaling




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