Description: Recombinant full-length human AURORA B was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Storage, Stability, and Shipping: Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Kinase Assay, Western Blot

Molecular Weight: ~68 kDa

Gene Aliases: AURKB, AIK2; AIM1; ARK2; AurB; IPL1; AIM-1; STK12

Scientific Background: AURORA B is a member of the Aurora kinase family that associates with microtubules during chromosome movement and segregation. AURORA B localizes to the microtubules near kinetochores, specifically to the specialized microtubules called K-fibers (1). AURORA B inhibits the microtubule depolymerizing activity of mitotic centromere-associated kinesin (MCAK) by phosphorylating MCAK on Ser92 (2). This phosphorylation also regulates MCAK translocalization from kinetochores to the centromere. AURORA B has been identified as a target for the development of new anticancer agents since inhibition of AURORA B gives rise to the more pronounced antiproliferative phenotype.

References: 
1. Shannon, K B. et al: Chromosome dynamics: new light on Aurora B kinase function. Curr Biol. 2002 Jul 9;12(13):R458-60.

2. Andrews, P. D. et al: Aurora B regulates MCAK at the mitotic centromere. Dev. Cell 6: 253-268, 2004.

		Specific Activity:  Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

		Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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