CDK3/CyclinE1 Active Enzymes Active Kinases


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CDK3/CyclinE1, Active (C30-10G)
Catalog Number Pack Size Price (USD)  
CDK3/CyclinE1, Active C30-10G-05 5 ug $205   CDK3/CyclinE1, Active Add to Cart
CDK3/CyclinE1, Active C30-10G-10 10 ug $295   CDK3/CyclinE1, Active Add to Cart
CDK3/CyclinE1, Active C30-10G-BULK BULK CONTACT US  
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Product Data Sheet(s)




Description: Recombinant full-length human CDK3 and Cyclin E1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
Tag: GST tags
Expression System: Sf9 insect cells using baculovirus
Species: Human
Sequence: Full length
Genbank Number: NM_001258 , NM_001238
   

Storage, Stability, and Shipping: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Kinase Assay, Western Blot

Molecular Weight: ~60 kDa and CyclinE1 ~73 kDa

Gene Aliases : CDK3: None CyclinE1: CCNE1, CCNE

Scientific Background: CDK3/CyclinE1is a member of the cyclin-dependent protein kinase family and promotes entry into S phase, in part by activating members of the E2F family of transcription factors. CDK3 can also associate with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. The CDK3 gene has been mapped to chromosomal location of 17q22-qter, telomeric to the BRCA1 gene by somatic cell hybrids analysis (1). The presence of a single point mutation in the CDK3 gene from several Mus musculus strains commonly used in the laboratory has been reported (2).

References: 
1. Bullrich, F. et al: Chromosomal mapping of members of the cdc2 family of protein kinases, cdk3, cdk6, PISSLRE, and PITALRE, and a cdk inhibitor, p27-Kip1, to regions involved in human cancer. Cancer Res. 55: 1199-1205, 1995.

2. Ye, X. et al: A premature-termination mutation in the Mus musculus cyclin-dependent kinase 3 gene. Proc. Nat. Acad. Sci. 98: 1682-1686, 2001.



  Specific Activity:  Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.


  Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.




CDK3/CyclinE1, Active Research Areas

The CDK3/CyclinE1, Active product can be utilized in the following research areas, but not limited to:

Cancer, Cell Cycle, Ser/Thr Kinases



CDK3/CyclinE1, Active Related Products Found: Total 61 Related Products
Product Type Product Name Catalog Number Application Pack Sizes
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Signaling Reagents Kinase Assay Buffer I K01-09 Kinase Assay View Kinase Assay Buffer I
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Enzymes ABL2, Active A04-11H Kinase Assay, Western Blot View ABL2, Active
Signaling Proteins CDK4, Unactive C31-14G Kinase Assay, Western Blot View CDK4, Unactive
Signaling Proteins CDK5, Unactive C33-14G Kinase Assay, Western Blot View CDK5, Unactive
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Signaling Proteins p21CIP1 Protein C27-30G Kinase Assay, Western Blot View p21CIP1 Protein
Signaling Proteins p27KIP1 Protein C28-30G Kinase Assay, Western Blot View p27KIP1 Protein
Signaling Reagents Rb (N-Term) Protein R05-56G Kinase Assay, Western Blot View Rb (N-Term) Protein
Enzymes TAOK1, Active T24-11G Kinase Assay, Western Blot View TAOK1, Active
Enzymes TAOK2, Active T25-11G Kinase Assay, Western Blot View TAOK2, Active
Enzymes CLK1, Active C57-11G Kinase Assay, Western Blot View CLK1, Active
Enzymes CLK2, Active C58-11G Kinase Assay, Western Blot View CLK2, Active
Enzymes CDK1/CyclinA2, Active C22-18G Kinase Assay, Western Blot View CDK1/CyclinA2, Active
Enzymes CDK7/Cyclin H1/MNAT1, Active C36-10H Kinase Assay, Western Blot View CDK7/Cyclin H1/MNAT1, Active
Signaling Proteins CDK1, Unactive C22-14G Kinase Assay, Western Blot View CDK1, Unactive
Signaling Reagents Kinase Dilution Buffer III K23-09 Kinase Assay View Kinase Dilution Buffer III
Signaling Proteins BCR Protein B01-35G Western Blot View BCR Protein
Signaling Proteins CDKN3 Protein C01-34G Western Blot View CDKN3 Protein
Signaling Proteins LATS2, Unactive L02-35G Kinase Assay, Western Blot View LATS2, Unactive
Signaling Proteins MDM2 Protein M45-31G Western Blot View MDM2 Protein
Signaling Proteins Tau-352 Protein T03-54N Kinase Assay, Western Blot View Tau-352 Protein
Signaling Proteins Tau-381 Protein T04-54N Kinase Assay, Western Blot View Tau-381 Protein
Signaling Proteins Tau-383 Protein T05-54N Kinase Assay, Western Blot View Tau-383 Protein
Signaling Proteins Tau-410 Protein T06-54N Kinase Assay, Western Blot View Tau-410 Protein
Signaling Proteins Tau-412 Protein T07-54N Kinase Assay, Western Blot View Tau-412 Protein
Signaling Proteins Tau-441 Protein T08-54N Kinase Assay, Western Blot View Tau-441 Protein
Enzymes CDK9/CyclinK, Active C40-10G Kinase Assay, Western Blot View CDK9/CyclinK, Active
Enzymes ABL1, Active A03-18H Kinase Assay, Western Blot View ABL1, Active
Signaling Proteins p18INK4C Protein C32-30G Kinase Assay, Western Blot View p18INK4C Protein
Signaling Proteins p19INK4D Protein C33-30G Kinase Assay, Western Blot View p19INK4D Protein
Signaling Proteins CDK6/CyclinD3, Unactive C35-14H Kinase Assay, Western Blot View CDK6/CyclinD3, Unactive
Enzymes CDK4/CyclinD3, Active C31-18G Kinase Assay, Western Blot View CDK4/CyclinD3, Active
Enzymes CDK6/CyclinD3, Active C35-10H Kinase Assay, Western Blot View CDK6/CyclinD3, Active
Signaling Reagents Rb (C-Term) Protein R05-55G Kinase Assay, Western Blot View Rb (C-Term) Protein
Signaling Proteins CDK9, Unactive C40-14G Kinase Assay, Western Blot View CDK9, Unactive
Signaling Proteins CDK6, Unactive C35-15H Kinase Assay, Western Blot View CDK6, Unactive
Signaling Proteins CDK2/CyclinE1, Unactive C29-14G Kinase Assay, Western Blot View CDK2/CyclinE1, Unactive
Signaling Proteins CDK8, Unactive C38-14G Kinase Assay, Western Blot View CDK8, Unactive
Enzymes CDK5/p35, Active C33-10BG Kinase Assay, Western Blot View CDK5/p35, Active
Antibodies Anti-CDC25A C04-63R WB View Anti-CDC25A
Signaling Reagents CDKtide C06-58 Kinase Assay View CDKtide
Enzymes CDK1/CyclinA1, Active C22-18BG Kinase Assay, Western Blot View CDK1/CyclinA1, Active
Enzymes CDK1/CyclinB1, Active C22-10G Kinase Assay, Western Blot View CDK1/CyclinB1, Active
Enzymes CDK2/CyclinA1, Active C29-10BG Kinase Assay, Western Blot View CDK2/CyclinA1, Active
Enzymes CDC7/DBF4, Active C26-10G Kinase Assay, Western Blot View CDC7/DBF4, Active
Signaling Proteins CDC7 Protein C26-30G Western Blot View CDC7 Protein
Signaling Proteins CDK2 Protein C29-30G Western Blot View CDK2 Protein
Signaling Proteins CDK3, Unactive C30-14G Kinase Assay, Western Blot View CDK3, Unactive
Signaling Proteins CyclinE1 Protein C82-30G Kinase Assay, Western Blot View CyclinE1 Protein
Signaling Proteins CyclinD1 Protein C81-30BG Kinase Assay, Western Blot View CyclinD1 Protein
Signaling Proteins CDK2, Unactive C29-14BG Kinase Assay, Western Blot View CDK2, Unactive
Signaling Proteins CDK4, Unactive C31-14BG Kinase Assay, Western Blot View CDK4, Unactive
Signaling Proteins CDK7, Unactive C36-14G Kinase Assay, Western Blot View CDK7, Unactive
Signaling Proteins CDK11, Unactive C42-14G Kinase Assay, Western Blot View CDK11, Unactive
Signaling Proteins CyclinC Protein C80-30G Western Blot View CyclinC Protein
Signaling Proteins CDK11B (CDC2L1) Protein New Signaling Proteins C23-30G Western Blot View CDK11B (CDC2L1) Protein
Signaling Proteins CyclinH Protein New Signaling Proteins C83-30H Western Blot View CyclinH Protein
Signaling Proteins CyclinO Protein New Signaling Proteins C85-30G Western Blot View CyclinO Protein
Signaling Proteins CyclinY Protein New Signaling Proteins C87-30G Western Blot View CyclinY Protein


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Product Types
Enzymes
Enzymes Active Acetyltransferases Active Acetyltransferases
Enzymes Active Arginine Deiminases Active Arginine Deiminases
Enzymes Active Histone Deacetylases Active Histone Deacetylases
Enzymes Active Kinases Active Kinases
Enzymes Active Methyltransferase Active Methyltransferase
Enzymes Active Phosphatases Active Phosphatases
Enzymes Active Phosphodiesterases Active Phosphodiesterases
Extracellular Ligands
Extracellular Ligands Chemokines Chemokines
Extracellular Ligands Cytokines Cytokines
Extracellular Ligands Growth Factors Growth Factors
Signaling Proteins
Signaling Proteins Acetyl/Methyltransferase Proteins Acetyl/Methyltransferase Proteins
Signaling Proteins Adaptor Proteins Adaptor Proteins
Signaling Proteins Apoptosis Proteins Apoptosis Proteins
Signaling Proteins Arginine Deiminases Arginine Deiminases
Signaling Proteins Cell Cycle Proteins Cell Cycle Proteins
Signaling Proteins Cell Stress & Chaperone Proteins Cell Stress & Chaperone Proteins
Signaling Proteins Cellular Proteins Cellular Proteins
Signaling Proteins Deacetylase/Demethylase Proteins Deacetylase/Demethylase Proteins
Signaling Proteins Fructose Kinase Proteins Fructose Kinase Proteins
Signaling Proteins G-Proteins G-Proteins
Signaling Proteins Lysyl Oxidase Proteins Lysyl Oxidase Proteins
Signaling Proteins Methylcytosine Dioxygenase Proteins Methylcytosine Dioxygenase Proteins
Signaling Proteins Microtubule/Actin Associated Proteins Microtubule/Actin Associated Proteins
Signaling Proteins Tau Proteins Tau Proteins
Signaling Proteins Transcription Proteins Transcription Proteins
Signaling Proteins Ubiquitin Proteins Ubiquitin Proteins
Signaling Proteins Unactive Kinases Unactive Kinases
Signaling Reagents
Signaling Reagents Assay Reagents Assay Reagents
Signaling Reagents Oligo Substrates Oligo Substrates
Signaling Reagents Peptide Substrates Peptide Substrates
Signaling Reagents Protein Substrates Protein Substrates
Antibodies
Antibodies Isoform Specific Antibodies Isoform Specific Antibodies
Antibodies Modification Antibodies Modification Antibodies
Antibodies Phospho Specific Antibodies Phospho Specific Antibodies
Antibodies Primary Antibodies Primary Antibodies
Antibodies Secondary Antibodies Secondary Antibodies
Antibodies Tag Antibodies Tag Antibodies
Discovery Services
Compound Selective Profiling Compound Selective Profiling
Custom Protein Development Custom Protein Development

Research Categories
AKT/PKB Pathway AKT/PKB Pathway
Angiogenesis Angiogenesis
Apoptosis/Autophagy Apoptosis/Autophagy
Cancer Cancer
Cardiovascular Disease Cardiovascular Disease
Cell Cycle Cell Cycle
Cellular Stress Cellular Stress
Cytoplasmic Tyrosine Kinases Cytoplasmic Tyrosine Kinases
ERK/MAPK Pathway ERK/MAPK Pathway
Inflammation Inflammation
Invasion/Metastasis Invasion/Metastasis
JAK/STAT Pathway JAK/STAT Pathway
JNK/SAPK Pathway JNK/SAPK Pathway
Lipid Kinases Lipid Kinases
Metabolic Disorder Metabolic Disorder
Neurobiology Neurobiology
NfkB Pathway NfkB Pathway
p38 Pathway p38 Pathway
Phosphatases Phosphatases
Phosphodiesterases Phosphodiesterases
PKA/PKC Pathway PKA/PKC Pathway
Receptor Tyrosine Kinases Receptor Tyrosine Kinases
Ser/Thr Kinases Ser/Thr Kinases
WNT Signaling WNT Signaling




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