Description: Recombinant human EPHB4 (561-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Storage, Stability, and Shipping: Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Kinase Assay, Western Blot

Molecular Weight: ~50 kDa

Gene Aliases : HTK, MYK1, TYRO11, Mdk2

Scientific Background: EPHB4 is a member of the Eph family of receptor tyrosine kinases which play a role in regulating cell adhesion and cell movement during embryonic development (1). Furthermore, because tissue disorganization and abnormal cell adhesion, movement, and survival characterize the more advanced stages of cancer, the inappropriate functioning of Eph receptor could play a causal role in malignancy (2). EPHB4 is uniquely expressed in the vascular endothelial and endocardial cells and animal knockout studies with EPHB4 reveal a phenotype that is similar to Ephrin B2 gene knockdown. Furthermore, EPHB4 has been shown to be the major essential interaction partner of Ephrin B2 in angiogenesis.

References: 
1. Andres, A.C. et al: Expression of two novel eph-related receptor protein tyrosine kinases in mammary gland development and carcinogenesis. Oncogene, 1994;9(8):2431.

2. Kumar, S.R. et al: The receptor tyrosine kinase EphB4 is overexpressed in ovarian cancer, provides survival signals and predicts poor outcome. Br. J. Cancer. 2007, 96(7):1083-91.

		Specific Activity:  Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

		Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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