Description: Recombinant full-length human PDHK4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Storage, Stability, and Shipping: Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Kinase Assay, Western Blot

Molecular Weight: ~71 kDa

Gene Aliases : PDK4; FLJ40832

Scientific Background: PDHK4 or Pyruvate Dehydrogenase Kinase 4 is a member of the PDHK family that phosphorylate and inactivate the Pyruvate Dehydrogenase (PDH) (1). PDH is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. PDHK4 upregulation in adipocytes participates in the hypolipidemic effect of thiazolidinediones through modulation of glyceroneogenesis (2). PDHK4 expression is strongly and selectively induced by Rosiglitazone in a direct and transcriptional manner.

References: 
1. Gudi, R. et al: Diversity of the pyruvate dehydrogenase kinase gene family in humans. J. Biol. Chem. 270: 28989-28994, 1995.

2. Cadoudal, T. et al: Pyruvate dehydrogenase kinase 4: regulation by thiazolidinediones and implication in glyceroneogenesis in adipose tissue. Diabetes.2008 Sep;57(9):2272-9.

		Specific Activity:  Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

		Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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