Description: Recombinant full-length human PI3K (p110 alpha/p55 gamma) was co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Storage, Stability, and Shipping: Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Kinase Assay, Western Blot

Molecular Weight: ~111 kDa (p110α), ~61 kDa (p85g)

Gene Aliases: p110a: PI3K, p110-alpha p55g: PIK3R3, p55, p55-GAMMA, FLJ41892

Scientific Background: PI3K (p110alpha/p55gamma) or Phosphatidylinositol 3-kinase is a lipid kinase that phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position which serve as second messengers in growth signaling pathways. PI3K comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in the PI3K has been associated with insulin resistance and plays an important role in glucose homeostasis in vivo (1). PI3K also plays an essential role in the development and induction of mast cells in normal and pathogenic immune responses (2).

References: 
1. Terauchi, Y. et.al: Increased insulin sensitivity and hypoglycaemia in mice lacking the p85-alpha subunit of phosphoinositide 3-kinase. Nature Genet. 21: 230-235, 1999.

2. Fukao, T. et.al: Selective loss of gastrointestinal mast cells and impaired immunity in PI3K-deficient mice. Nature Immun. 3: 295-304, 2002.

		Specific Activity:  Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

		Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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