Description: Recombinant full-length human PKAcalpha was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Storage, Stability, and Shipping: Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Kinase Assay, Western Blot

Molecular Weight: ~69 kDa

Gene Aliases : PKAa; cAPKa; MGC48865; MGC102831

Scientific Background: The catalytic subunit C-alpha of PKA (PKAcα) is a member of the Ser/Thr protein kinase family and has been assigned to chromosome region 19p13.1 (1). Null mutation in PKAcα leads to early postnatal lethality in the majority of C-alpha knockout mice. Surprisingly, a small percentage of C-alpha knockout mice, although runted, survived to adulthood. In these animals, compensatory increases in C-beta levels occurred in brain whereas many tissues, including skeletal muscle, heart, and sperm, contained less than 10% of the normal PKA activity (2).

References: 
1. Tasken, K. et al: The gene encoding the catalytic subunit C-alpha of cAMP-dependent protein kinase (locus PRKACA) localizes to human chromosome region 19p13.1. Genomics 36: 535-538, 1996.

2. Skalhegg, BS. Et al: Mutation of the C-alpha subunit of PKA leads to growth retardation and sperm dysfunction. Molec. Endocr. 16: 630-639, 2002.

		Specific Activity:  Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

		Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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