Active Proteases


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SignalChem's Active Proteases

Proteases (or peptidases) are proteolytic enzymes that hydrolyze the peptide bonds between amino acids. Endoproteases are proteases that cleave intramolecular linkages within proteins, making these types of enzymes important tools for industrial processes and biochemical research. TEV Protease and Thrombin are examples of endoproteases that target well-defined consensus sequences, a property which makes them useful for removing affinity tags and for cleaving fusion proteins. In contrast, promiscuous proteases such as trypsin cut peptides more frequently. Trypsin targets peptide bonds adjacent to lysine and arginine residues, enabling researchers to digest proteins into discriminate fragments in preparation for following steps such as peptide mapping.

Endoproteases are also critical for cleaving amino acid linkages to interconvert prodrugs to their parental drugs. For example, Trypsin and Lysyl-Endopeptidase (Lys-c) are involved in removal of the C-peptide from pro-insulin, a necessary step towards the production of insulin.

To facilitate production processes that require large-scale proteolysis steps, Immobilized Proteases offer several advantages, which include:

Cost Savings – the immobilized enzyme resin can be reused up to 20 times
Protocol Flexibility – immobilized proteases are amenable to suspension or in-column digestion protocols
Higher Product Yield and Quality – enzyme separation steps are eliminated

SignalChem’s Protease Enzymes are fully characterized for their activity and specificity. All products, including agarose and/or silica cross-linked proteases, are available in microgram to gram quantities.


Active Proteases Product Listing Found: Total 12 Products Matching Active Proteases
Product Name Catalog Number Species Tag Expression System Sequence
CPB (carboxypeptidase B), Active C560-31N Rat tag-free Yeast cells 109-end
EK (enterokinase), Active E535-31H Swine His tag Yeast cells 800-end
Immobilized Lys-c, Agarose L585-31AN Bacteria tag-free E.coli 206-473 aa
Immobilized Lys-c, Silica L585-31SN Bacteria tag-free E.coli 206-473 aa
Immobilized Thrombin, Agarose T565-31AN Human Native
Immobilized Trypsin, Agarose T575-31AN Swine tag-free Yeast cells 10-end
Immobilized Trypsin, Silica T575-31SN Swine tag-free Yeast cells 10-end
KEX2 Protease, Active K525-31N Yeast tag-free Yeast cells 110-660 aa
Lys-c (lysyl-endopeptidase), Active L585-31N Bacteria tag-free E.coli 206-473 aa
TEV (Tobacco Etch Virus) Protease, Active T525-31H TE Virus His tag E.coli 2038-2279 aa
Thrombin, Active T565-31N Human tag-free E.coli 364-end
Trypsin, Active T575-31N Swine tag-free Yeast cells 10-end
Active Proteases Product Listing Found: Total 12 Products Matching Active Proteases
Product Name Catalog Number Species Tag Expression System Sequence
CPB (carboxypeptidase B), Active C560-31N Rat tag-free Yeast cells 109-end
Active Proteases Product Listing Found: Total 12 Products Matching Active Proteases
Product Name Catalog Number Species Tag Expression System Sequence
EK (enterokinase), Active E535-31H Swine His tag Yeast cells 800-end
Active Proteases Product Listing Found: Total 12 Products Matching Active Proteases
Product Name Catalog Number Species Tag Expression System Sequence
Immobilized Lys-c, Agarose L585-31AN Bacteria tag-free E.coli 206-473 aa
Immobilized Lys-c, Silica L585-31SN Bacteria tag-free E.coli 206-473 aa
Immobilized Thrombin, Agarose T565-31AN Human Native
Immobilized Trypsin, Agarose T575-31AN Swine tag-free Yeast cells 10-end
Immobilized Trypsin, Silica T575-31SN Swine tag-free Yeast cells 10-end
Active Proteases Product Listing Found: Total 12 Products Matching Active Proteases
Product Name Catalog Number Species Tag Expression System Sequence
KEX2 Protease, Active K525-31N Yeast tag-free Yeast cells 110-660 aa
Lys-c (lysyl-endopeptidase), Active L585-31N Bacteria tag-free E.coli 206-473 aa
Active Proteases Product Listing Found: Total 12 Products Matching Active Proteases
Product Name Catalog Number Species Tag Expression System Sequence
Active Proteases Product Listing Found: Total 12 Products Matching Active Proteases
Product Name Catalog Number Species Tag Expression System Sequence
Active Proteases Product Listing Found: Total 12 Products Matching Active Proteases
Product Name Catalog Number Species Tag Expression System Sequence
TEV (Tobacco Etch Virus) Protease, Active T525-31H TE Virus His tag E.coli 2038-2279 aa
Thrombin, Active T565-31N Human tag-free E.coli 364-end
Trypsin, Active T575-31N Swine tag-free Yeast cells 10-end
Active Proteases Product Listing Found: Total 12 Products Matching Active Proteases
Product Name Catalog Number Species Tag Expression System Sequence
Active Proteases Product Listing Found: Total 12 Products Matching Active Proteases
Product Name Catalog Number Species Tag Expression System Sequence
Active Proteases Product Listing Found: Total 12 Products Matching Active Proteases
Product Name Catalog Number Species Tag Expression System Sequence
Active Proteases Product Listing Found: Total 12 Products Matching Active Proteases
Product Name Catalog Number Species Tag Expression System Sequence


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Product Types
Enzymes
Enzymes Active Acetyltransferases Active Acetyltransferases
Enzymes Active Arginine Deiminases Active Arginine Deiminases
Enzymes Active GTPases/ATPases Active GTPases/ATPases
Enzymes Active Histone Deacetylases Active Histone Deacetylases
Enzymes Active Kinase Mutants Active Kinase Mutants
Enzymes Active Kinases Active Kinases
Enzymes Active Lipid Kinases Active Lipid Kinases
Enzymes Active Methyltransferases Active Methyltransferases
Enzymes Active Phosphatases Active Phosphatases
Enzymes Active Phosphodiesterases Active Phosphodiesterases
Enzymes Active Proteases Active Proteases
Antibodies
Antibodies Isoform Specific Antibodies Isoform Specific Antibodies
Antibodies Modification Antibodies Modification Antibodies
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Antibodies Primary Antibodies Primary Antibodies
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Antibodies Tag Antibodies Tag Antibodies
Biochemical Compounds
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Biochemical Compounds Kinase Activators Kinase Activators
Biochemical Compounds Kinase Inhibitors Kinase Inhibitors
Biochemical Compounds Phosphatase Inhibitors Phosphatase Inhibitors
Biochemical Compounds siRNA Controls siRNA Controls
Biochemical Compounds siRNA Sets siRNA Sets
Signaling Proteins
Signaling Proteins Acetyl/Methyltransferases Acetyl/Methyltransferases
Signaling Proteins Adaptor Proteins Adaptor Proteins
Signaling Proteins Apoptosis Proteins Apoptosis Proteins
Signaling Proteins Arginine Deiminases Arginine Deiminases
Signaling Proteins Cell Cycle Proteins Cell Cycle Proteins
Signaling Proteins Cell Stress & Chaperone Proteins Cell Stress & Chaperone Proteins
Signaling Proteins Cellular Proteins Cellular Proteins
Signaling Proteins Deacetylase/Demethylases Deacetylase/Demethylases
Signaling Proteins Dihydropyrimidinase Dihydropyrimidinase
Signaling Proteins Dioxygenases Dioxygenases
Signaling Proteins Fructose Kinases Fructose Kinases
Signaling Proteins G-Proteins G-Proteins
Signaling Proteins Kinase Mutant Proteins Kinase Mutant Proteins
Signaling Proteins Kinase Protein Kinase Protein
Signaling Proteins Lysyl Oxidases Lysyl Oxidases
Signaling Proteins Microtubule/Actin Associated Proteins Microtubule/Actin Associated Proteins
Signaling Proteins Phospholipase C Proteins Phospholipase C Proteins
Signaling Proteins Superoxide Dismutase Superoxide Dismutase
Signaling Proteins Tau Proteins Tau Proteins
Signaling Proteins Transcription Proteins Transcription Proteins
Signaling Proteins Ubiquitin Proteins Ubiquitin Proteins
Signaling Proteins Unactive Kinases Unactive Kinases
Signaling Reagents
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Signaling Reagents Lipid Substrates Lipid Substrates
Signaling Reagents Oligo Substrates Oligo Substrates
Signaling Reagents Peptide Substrates Peptide Substrates
Signaling Reagents Protein Substrates Protein Substrates
Extracellular Ligands
Extracellular Ligands Chemokines Chemokines
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Research Categories
AKT/PKB Pathway AKT/PKB Pathway
Angiogenesis Angiogenesis
Apoptosis/Autophagy Apoptosis/Autophagy
Cancer Cancer
Cardiovascular Disease Cardiovascular Disease
Cell Cycle Cell Cycle
Cellular Stress Cellular Stress
Cytoplasmic Tyrosine Kinases Cytoplasmic Tyrosine Kinases
ERK/MAPK Pathway ERK/MAPK Pathway
Inflammation Inflammation
Invasion/Metastasis Invasion/Metastasis
JAK/STAT Pathway JAK/STAT Pathway
JNK/SAPK Pathway JNK/SAPK Pathway
Lipid Kinases Lipid Kinases
Metabolic Disorder Metabolic Disorder
Neurobiology Neurobiology
NfkB Pathway NfkB Pathway
p38 Pathway p38 Pathway
Phosphatases Phosphatases
Phosphodiesterases Phosphodiesterases
PKA/PKC Pathway PKA/PKC Pathway
Receptor Tyrosine Kinases Receptor Tyrosine Kinases
Ser/Thr Kinases Ser/Thr Kinases
WNT Signaling WNT Signaling




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