Description: Rabbit Polyclonal Antibody

Specificity: Recognizes the AKT1, AKT2, and AKT3 protein

Cited Applications: WB

Cross Reactivity: Western blot AKT1, AKT2, and AKT3 from human and mouse cells

AKT1, AKT2, and AKT3 from other species may also be detectable

Host : Rabbit

Immunogen: Synthetic peptide corresponding to amino acids 313-317 of human AKT1 (protein accession number P31749)

Formulation: PBS pH 7.4, 0.02% sodium azide, 50% glycerol

Purification: Affinity chromatography

Stability: Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473 (1).

References: 1. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.

		Sample Data:  Representative western blot with Anti-AKT (pan) (1:1000) using 15 ng of human recombinant AKT1, AKT2, and AKT3.

		Sample Data:  Representative western blot with Anti-AKT (pan) (1:1000) using 3 μg, 10 μg and 30 μg of 3T6 cell lysate.

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