Description: Rabbit Polyclonal Antibody

Specificity: Anti-ERK2 antibody recognizes the ERK2 protein.

Cited Applications: WB

Cross Reactivity: ERK2 from human, rat, bovine, mouse and rabbit

ERK2 from other species may also be detectable.

Host: Rabbit

Immunogen: Human ERK2 c-terminal peptide-KLH conjugates

Formulation: PBS at pH 7.2, 50% glycerol

Purification: Affinity purified by ERK2 c-terminal peptide on agarose from rabbit serum

Stability: Store at 4°C (add 0.1% NaN₃) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: ERK2 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK2 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK2 is ubiquitously distributed in tissues with the highest expression in heart, brain and spinal cord. Activated ERK2 translocates into the nucleus where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos, and C/EBP beta).

References: 
1. Boulton, TG. et al: Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase. Biochemistry. 1991 Jan 8;30(1):278-86.

		Sample Data:  Representative western blot from 293 cell lysate using ERK2 antibody (1:2500).

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