: Recognizes the VAV protein
: Western blot VAV from human cells
VAV from other species may also be detectable
: Synthetic peptide corresponding to amino acids 172-176 of human VAV
: PBS, pH 7.4, 0.02% NaN3, 50% glycerol
: Affinity chromatography
: Store at 4o
C (add 0.1% NaN3
) for several months, and at -20o
C for longer periods.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
: VAV is a proto-oncogene that is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. VAV plays a vital role in hematopoiesis and in T-cell and B-cell development as well as in their activation. VAV is the specific binding partner of Nef proteins from HIV-1 (1). Co-expression and binding of these VAV partners initiates profound morphological changes, cytoskeletal rearrangements and JNK/SAPK pathway activation leading to increased levels of viral transcription and replication. VAV is also the guanine nucleotide dissociation inhibitor for Ras.
1. Fackler, O T. et al: Activation of Vav by Nef induces cytoskeletal rearrangements and downstream effector functions. Molec. Cell 3729-739, 1999.
||Sample Data : Representative Immunoprecipitation with 2 μg of Anti-VAV using 500 μg of Jurkat and THP1cell lysates.
||Sample Data : Representative Western blot with Anti-VAV (1:1000) using 3 μg, 10 μg and 30 μg of Jurkat cell lysate.
The Anti-VAV product can be utilized in the following research areas, but not limited to:
Apoptosis/Autophagy, ERK/MAPK Pathway, JNK/SAPK Pathway, p38 Pathway