Description: Rabbit Polyclonal Antibody

Specificity: Recognizes the VAV protein

Cited Applications: WB,IP

Cross Reactivity: Western blot VAV from human cells

VAV from other species may also be detectable

Host : Rabbit

Immunogen: Synthetic peptide corresponding to amino acids 172-176 of human VAV

Formulation: PBS, pH 7.4, 0.02% NaN3, 50% glycerol

Purification: Affinity chromatography

Stability: Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: VAV is a proto-oncogene that is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. VAV plays a vital role in hematopoiesis and in T-cell and B-cell development as well as in their activation. VAV is the specific binding partner of Nef proteins from HIV-1 (1). Co-expression and binding of these VAV partners initiates profound morphological changes, cytoskeletal rearrangements and JNK/SAPK pathway activation leading to increased levels of viral transcription and replication. VAV is also the guanine nucleotide dissociation inhibitor for Ras.

References: 
1. Fackler, O T. et al: Activation of Vav by Nef induces cytoskeletal rearrangements and downstream effector functions. Molec. Cell 3729-739, 1999.

		Sample Data :  Representative Immunoprecipitation with 2 μg of Anti-VAV using 500 μg of Jurkat and THP1cell lysates.

		Sample Data :  Representative Western blot with Anti-VAV (1:1000) using 3 μg, 10 μg and 30 μg of Jurkat cell lysate.

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