Description: Recombinant full-length human XIAP was expressed by baculovirus in Sf9 insect cells using a C-terminal GST tag.

Storage, Stability, and Shipping: Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Western Blot

Molecular Weight: ~84 kDa

Gene Aliases: API3, ILP1, MIHA, XLP2, BIRC4

Scientific Background: XIAP is a member of a family of proteins which inhibit apoptosis and act as mammalian cell-death suppressors. XIAP inhibits at least 2 members of the caspase family of cell-death proteases, caspase-3 and caspase-7 (1). In addition, XIAP inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE. Furthermore, XIAP can mediate protection of cells from apoptosis by utilizing both a JNK1 activation pathway that involves ILPIP and a caspase inhibition pathway that is independent of ILPIP (2). Disruption of the XIAP gene in human colon cancer cells can enhance sensitivity of these cells to exogenously added TRAIL suggesting that XIAP is a nonredundant modulator of TRAIL-mediated apoptosis.

References: 
1. Deveraux Q L, et al: X-linked X-linked IAP is a direct inhibitor of cell-death proteases. Nature 388: 300-304, 1997.

2. Tang G, et al: Inhibition of JNK activation through NF- kappa-B target genes. Nature 414: 313-317, 2001.

		Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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